Development of Integrated Electrochemical–QuartzCrystal Microbalance Biosensor Arrays: Towards Ultrasensitive, Multiplexed and Rapid Point-of-Care Dengue Detection

Ahmad Zainuddin, Anis Nordin, Mohd Asri, Rosminazuin Rahim, Cyril Guines, Matthieu Chatras, Arnaud Pothier, Wing Mak

Abstract

Dengue is an infectious mosquito-borne viral disease that affects approximately 50 million people annually worldwide and is prevalent mostly in the tropics. Severe cases of dengue can be fatal, making early detection and fast diagnosis crucial towards improving patient care and survival rates. Currently, early detection can be achieved through detection of NS1 protein, using ELISA technique. Unfortunately, ELISA is an expensive method, making it unsuitable as a screening technique, especially in low-resource settings. In this work, we present a prototype device and its early validation studies, of an integrated electrochemical and mass-sensor for dengue NS1 antigen. The sensor is connected to open source mass-sensing software and hardware, OpenQCM which makes it easily portable. Having dual-measurement capabilities (mass and impedance) increases the sensitivity of the sensor. Preliminary studies suggest that the prototype could achieve ultralow limit of detection as low as 10 ng mL-1, dual-sensing cross-validation capability, portable size, time of less than 30 minutes, and parallelization of multiple assays. This work could lead to early and accurate dengue detection in routine point-of-care settings.

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in Harvard Style

Zainuddin A., Nordin A., Asri M., Rahim R., Guines C., Chatras M., Pothier A. and Mak W. (2019). Development of Integrated Electrochemical–QuartzCrystal Microbalance Biosensor Arrays: Towards Ultrasensitive, Multiplexed and Rapid Point-of-Care Dengue Detection.In Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, ISBN 978-989-758-353-7, pages 220-227. DOI: 10.5220/0007523802200227


in Bibtex Style

@conference{biodevices19,
author={Ahmad Zainuddin and Anis Nordin and Mohd Asri and Rosminazuin Rahim and Cyril Guines and Matthieu Chatras and Arnaud Pothier and Wing Mak},
title={Development of Integrated Electrochemical–QuartzCrystal Microbalance Biosensor Arrays: Towards Ultrasensitive, Multiplexed and Rapid Point-of-Care Dengue Detection},
booktitle={Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES,},
year={2019},
pages={220-227},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007523802200227},
isbn={978-989-758-353-7},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES,
TI - Development of Integrated Electrochemical–QuartzCrystal Microbalance Biosensor Arrays: Towards Ultrasensitive, Multiplexed and Rapid Point-of-Care Dengue Detection
SN - 978-989-758-353-7
AU - Zainuddin A.
AU - Nordin A.
AU - Asri M.
AU - Rahim R.
AU - Guines C.
AU - Chatras M.
AU - Pothier A.
AU - Mak W.
PY - 2019
SP - 220
EP - 227
DO - 10.5220/0007523802200227