NITRITE BIOSENSING WITH DISPOSABLE ELECTRODE STRIPS - A Preliminary Study

Marcelo Rodrigues, Cátia Correia, Célia M. Silveira, José J. G. Moura, Estibaliz Ochoteco, Elena Jubete, M. Gabriela Almeida

2012

Abstract

This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40C or 60C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.M-1.cm-2 within a linear range of 0.001 - 1 mM.

References

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Paper Citation


in Harvard Style

Rodrigues M., Correia C., M. Silveira C., J. G. Moura J., Ochoteco E., Jubete E. and Gabriela Almeida M. (2012). NITRITE BIOSENSING WITH DISPOSABLE ELECTRODE STRIPS - A Preliminary Study . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012) ISBN 978-989-8425-91-1, pages 32-36. DOI: 10.5220/0003757700320036


in Bibtex Style

@conference{biodevices12,
author={Marcelo Rodrigues and Cátia Correia and Célia M. Silveira and José J. G. Moura and Estibaliz Ochoteco and Elena Jubete and M. Gabriela Almeida},
title={NITRITE BIOSENSING WITH DISPOSABLE ELECTRODE STRIPS - A Preliminary Study},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)},
year={2012},
pages={32-36},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003757700320036},
isbn={978-989-8425-91-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)
TI - NITRITE BIOSENSING WITH DISPOSABLE ELECTRODE STRIPS - A Preliminary Study
SN - 978-989-8425-91-1
AU - Rodrigues M.
AU - Correia C.
AU - M. Silveira C.
AU - J. G. Moura J.
AU - Ochoteco E.
AU - Jubete E.
AU - Gabriela Almeida M.
PY - 2012
SP - 32
EP - 36
DO - 10.5220/0003757700320036