Carbon Electrode based Urea Sensor - Modification of Graphite and New Polymeric Carriers for Enzyme Immobilization

Julija Razumiene, Ieva Sakinyte, Tatjana Kochane, Sandra Maciulyte, Antanas Straksys, Saulute Budriene, Jurgis Barkauskas

2013

Abstract

The amperometric biosensor for urea determination was designed based on the electrochemical oxidation of urea decomposition products produced by urease. The enzyme electrode, made of a specially developed modified graphite (MG) paste, was produced by covering the electrode surface with new polymeric carriers poly(urethane-urea) (PUU) microparticles containing immobilized urease from Canavalia ensiformis (E.C. 3.5.1.5.).

References

  1. Agui, L., Yanes-Sedeno, P., Pingarron, J.M., (2008). Role of carbon nanotubes in electroanalytical chemistry: a review. Anal. Chim. Acta 622, 11-47.
  2. Budriene, S., Romaskevic, T., Pielichowski, K., Pielichowski, J., (2007). Synthesis and characterization of polyurethane microspheres and their application for immobilization of maltogenase. Polym. Adv. Technol., 18, 67-71.
  3. Fan, J., Yudasaka, M., Miyawaki, J., Ajima, K., Murata, K., Lijima, S., (2006). Control of hole opening in single-wall carbon nanotubes and single-wall carbon nanohorns using oxygen. J. Phys. Chem. B, 110, 1587- 1591.
  4. Miglior, F., Sewalem, A., Jamrozik, J., Bohmanova J., Lefebre, D. M., Motore, R. K., (2007). Genetic Analysis of Milk Urea Nitrogen and Lactose and Their Relationships with Other Production Traits in Canadian Holstein Cattle. J. Dairy Sc. 90, 2468-2479.
  5. Muti, M., Sharma, S. A. Erdem, Papakonstantinou, P., (2011). Electrochemical Monitoring of Nucleic Acid Hybridization by Single-Use Graphene Oxide-Based Sensor. Electroanalysis, 23 (1), 272-279.
  6. Patton, C. J., Crouch, S. R., (1977). Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia. Anal. Chem. 49, 464- 469.
  7. Randall, D. and Lee, S., (2002). The Polyurethane Book, John Wiley & Sons, New York, USA.
  8. Renny, E. F., Daniel, D. K., Krastanov, A. I., Zachariah, C. A., Elizabeth, R., (2005). Enzyme based sensor for detection of urea in milk. Biotechnol. & Biotechnol. Equip. 19 (2), 198-201.
  9. Voitechovic, E., Razumiene, E., Sakinyte, E., Barkauskas, J., (2010). Investigation of bioelectrocatalytic systems with PQQ-dependent GDH and carbonaceous materials. Biologija. 56. (1-4), 83-87.
  10. Wang, J., Kawde, A. N., Jan, M. R., (2004). Carbonnanotube-modified electrodes for amplified enzymebased electrical detection of DNA hybridization[J]. Biosens. Bioelectron. 20, 995-1000.
  11. Qureshi, A., Kang, W. P., Davidson, J. L., Gurbuz, Y., (2009). Review on carbon-derived, solid-state, micro and nano sensors for electrochemical sensing applications. Diamond & Related Materials, 18, 1401- 1420.
Download


Paper Citation


in Harvard Style

Razumiene J., Sakinyte I., Kochane T., Maciulyte S., Straksys A., Budriene S. and Barkauskas J. (2013). Carbon Electrode based Urea Sensor - Modification of Graphite and New Polymeric Carriers for Enzyme Immobilization . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013) ISBN 978-989-8565-34-1, pages 197-201. DOI: 10.5220/0004326901970201


in Bibtex Style

@conference{biodevices13,
author={Julija Razumiene and Ieva Sakinyte and Tatjana Kochane and Sandra Maciulyte and Antanas Straksys and Saulute Budriene and Jurgis Barkauskas},
title={Carbon Electrode based Urea Sensor - Modification of Graphite and New Polymeric Carriers for Enzyme Immobilization},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013)},
year={2013},
pages={197-201},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0004326901970201},
isbn={978-989-8565-34-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013)
TI - Carbon Electrode based Urea Sensor - Modification of Graphite and New Polymeric Carriers for Enzyme Immobilization
SN - 978-989-8565-34-1
AU - Razumiene J.
AU - Sakinyte I.
AU - Kochane T.
AU - Maciulyte S.
AU - Straksys A.
AU - Budriene S.
AU - Barkauskas J.
PY - 2013
SP - 197
EP - 201
DO - 10.5220/0004326901970201