EGFR-targeting Peptide Conjugated pH-sensitive Micelles as a Potential Drug Carrier for Photodynamic Detection and Therapy of Cancer

Cheng-Liang Peng, Yuan-I Chen, Ying-Hsia Shih, Tsai-Yueh Luo, Ming-Jium Shieh

2016

Abstract

Multifunctional theranostics have recently been intensively explored to optimize the efficacy and safety. Herein, we report multifunctional micelle that constructed from graft copolymer PEGMA-co-PDPA and diblock copolymer mPEG-b-PCL as the carrier of hydrophobic photosensitizer, chlorin e6 (Ce6) for simultaneous fluorescence imaging and photodynamic therapy. The functional inner core of PEGMA-co-PDPA exhibited pH stimulate to accelerate drug release under slightly acidic microenvironments of tumors and the outer shell of micelles with epidermal growth factor receptor (EGFR)-targeting GE11 peptides for active targeting of EGFR-overexpressing cancer cells. The results demonstrate that GE11-conjugated chlorin e6-loaded micelles (GE11-Ce6-micelles) with particle size around 100 nm and the micelles had well defined core shell structure which was evaluated by TEM. In the in vitro cellular uptake studies, GE11-Ce6-micelles exhibited a higher amount of intracellular uptake of chlorin e6 in HCT116 cancer cells (EGFR high expression) via receptor-mediated endocytosis, in contrast with the time-dependent passive uptake of the non-targeted Ce6-micelles, thereby providing a effective photocytotoxic effect on the HCT116 cancer cells. In vivo study revealed that GE11-Ce6-micelles exhibited tumor targeting for photodynamic detection and excellent inhibition on tumor growth after irradiation, indicating that GE11-Ce6-micelles could be successfully applied to the effective fluorescence imaging and photodynamic therapy of cancer.

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Paper Citation


in Harvard Style

Peng C., Chen Y., Shih Y., Luo T. and Shieh M. (2016). EGFR-targeting Peptide Conjugated pH-sensitive Micelles as a Potential Drug Carrier for Photodynamic Detection and Therapy of Cancer . In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 2: BIOIMAGING, (BIOSTEC 2016) ISBN 978-989-758-170-0, pages 105-110. DOI: 10.5220/0005774201050110


in Bibtex Style

@conference{bioimaging16,
author={Cheng-Liang Peng and Yuan-I Chen and Ying-Hsia Shih and Tsai-Yueh Luo and Ming-Jium Shieh},
title={EGFR-targeting Peptide Conjugated pH-sensitive Micelles as a Potential Drug Carrier for Photodynamic Detection and Therapy of Cancer},
booktitle={Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 2: BIOIMAGING, (BIOSTEC 2016)},
year={2016},
pages={105-110},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005774201050110},
isbn={978-989-758-170-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 2: BIOIMAGING, (BIOSTEC 2016)
TI - EGFR-targeting Peptide Conjugated pH-sensitive Micelles as a Potential Drug Carrier for Photodynamic Detection and Therapy of Cancer
SN - 978-989-758-170-0
AU - Peng C.
AU - Chen Y.
AU - Shih Y.
AU - Luo T.
AU - Shieh M.
PY - 2016
SP - 105
EP - 110
DO - 10.5220/0005774201050110