n-hexane, ethylacetate, ethanol fractions and
quercetine as positive control with DPPH assay
measured as IC
50
were 297.33 ± 0.46; 177.99 ± 0.32;
37.92 ± 1.03 and 2.32 ± 0.01 µg/mL respectively.
3.2 Inhibitory Concentration 50% (IC
50
)
MTT method was used to determine cell viability
after incubation for 24 h. In every treatment
fractions and doxorubicin was shown to inhibit cells
growth. The IC
50
from n-hexane, ethylacetate,
ethanol fractions and doxorubicin as positive control
were 327.89 ± 1.13; 64.92 ± 0.72; 1591.75 ± 37.05
and 1.82 ± 0.05 µg/mL respectively.The cytotoxicity
estimate of natural product is related to content of
active compound in these plants including Vernonia
amygdalina Del. Flavonoids and
triterpenoids/steroids estimated as active compounds
(Yadav, et al., 2012; Igile, et al., 1994; Quassie, et
al., 2016). Doxorubicin is one of chemotherapeutic
agent showed strong activity on T47D cell lines with
IC
50
value of 1.82 ± 0.05 µg/mL. T47D cells line
underwent resistant to doxorubicin pass through to
p53 mutation (Di Leo, et al., 2007; Vassade, et al.,
2005).
ACKNOWLEDGEMENTS
We gratefully thank to Directorate of Higher
Education, Ministry of Research Technology and
High Education, Indonesia through “Hibah
Penelitian Dasar Unggulan Perguruan Tinggi”
Research Grant 2018 for financial support in the
study.
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