Authors:
Nilam Anggraeni
1
;
Kristanti Wanito Wigati
2
;
I Lukitra Wardani
3
and
Lilik Herawati
2
Affiliations:
1
Medical Student Faculty of Medicine Universitas Airlangga, Indonesia
;
2
Department of Physiology, Faculty of Medicine Universitas Airlangga, Jalan Mayjen Prof. Dr. Moestopo no. 47, 60131 Surabaya and East Java, Indonesia
;
3
Physical Medicine and Rehabilitation Department Faculty of MedicineUniversitas Airlangga, Indonesia
Keyword(s):
high calorie, diet, hyperglycemia, astrocyte, cerebral cortex
Abstract:
Abstract: Nowadays, high-calorie food consumption people can unwittingly affect health. Several studies have reported the effects of excesshigh-calorie food consumption can range fromhyperglycaemia to neurodegenerative interference. In the brain, astrocytes function to respond to the changing modificationof molecular brain environment.structure. This study aimed to observe the effect of a high-calorie diet on the brain histology of male mice (Mus musculus). In this study, 28 mice were randomly divided into 4 groups: the control group pre (K0); the control group post with a standard diet (K1) ;the treatment group with 0.15g of glucose diet (K2); and the treatment group with 0.25g of glucose diet (K3). Treatment was given everyday for eightweeks. Brains were then histologically processed and stained with Haematoxylin-Eosin (HE) and observation made of quantitative changes of astrocytes in the cerebral cortex. Results: Data were analyzed with Post-Hoc ANOVA. From the study, significant
differences were found between K0 and K2 (α <0.05). In addition, significant differences were obtained in the groups K0 and K3 (α <0.05). Meanwhile, no significant differences were found between groups (α> 0.05). In conclusion, a high-calorie diet affects thee brain histology of male mice.
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