Authors:
Veena Tandon
1
;
Devendra Kumar Biswal
2
;
Pramod Kumar Prasad
1
and
Chenkual Malsawmtluangi
1
Affiliations:
1
North-Eastern Hill University, India
;
2
North Eastern Hill University, India
Keyword(s):
Mesocestoides, Cyclophyllidea Cestodes, ITS2 rDNA, Molecular Morphometrics, Sequence-Structure alignment.
Related
Ontology
Subjects/Areas/Topics:
Bioinformatics
;
Biomedical Engineering
;
Genomics and Proteomics
;
Sequence Analysis
;
Structural Bioinformatics
Abstract:
The phylogenetic relationships and systematic position of cyclophyllidean cestodes have always been controversial and opinions of different authors on the systematic rank and content of this order have varied greatly. Molecular phylogenetic analysis based on ITS2 rDNA of 16 representatives spanning 6 different families (Mesocestoididae, Davaineidae, Anoplocephalidae, Taeniidae, Dipylidiidae and Hymenolepididae) of the Order Cyclophyllidea and one out group from the family Diphyllobothriidae of the Order Pseudophyllidea confirmed the monophyletic nature of the Order Cyclophyllidea. Further, the results were validated by bayesian analysis, primary sequence-structure alignment and subsequent molecular morphometrics analysis. At the major nodes all the trees from various analyses were similar. Mesocestoides was interestingly accommodated within Cyclophyllidea and served as a sister clade close to the families Taeniidae, Anoplocephalidae, Hymenolepididae and Dipylidiidae.