Authors:
Ke Wu
;
Xiaoyan Qiu
and
Renrong Liu
Affiliation:
School of Life Science, Jiangxi Science & Technology Normal University, Jiangxi, Nanchang, China
Keyword(s):
Aflatoxin B1, Phage Display Peptide Library, Mimic Epitope, ELISA.
Abstract:
Aflatoxin B1 (AFB1) is a natural pollutant with strong toxicity and carcinogenicity. It not only causes huge economic losses, but also poses a serious threat to human, livestock and poultry health. To acquire the mimic epitope peptide of aflatoxin B1 and establish a non-toxic detection system of aflatoxin, an anti-afb13c7 monoclonal antibody was used as the target molecule, and the mimic epitopes of AFB1 were screened from the phage random 7 peptide library. A total of 39 phage clones were selected for verification. 36 of them could specifically bind to the antibody, and 30 of them could be inhibited by AFB1, whose DNA was extracted and sequenced. The results showed that 30 positive phage particles were actually 17 phage clones. The common sequence was histidine (H) - proline (P) - tryptophan (W), abbreviated as xxxxhpw, xxxhpwx, xxhpwxx, xhpwxxx (X was any amino acid). The linear range, detection limit and half inhibitory concentration (IC50) of the 17 positive phage particles were
similar. The linear range was 1-2000 pg / ml.
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