Authors:
Thianti Sylviningrum
1
;
Afifah
2
;
Dody Novrial
3
;
Brian Wasita
4
;
Bambang Purwanto
5
and
Harijono K. Sentono
6
Affiliations:
1
Doctoral Degree of Medical Science Programme, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia, Indonesia
;
2
Department of Pharmacology, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Indonesia
;
3
Department of Anatomical Pathology, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Indonesia
;
4
Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
;
5
Department of Internal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
;
6
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Keyword(s):
Imiquimod, skin, mice model, psoriasis.
Abstract:
Psoriasis is an incurable inflammatory disease with erythematous, scaly, and thick skin. Imiquimod (IMQ)-induced psoriasis mice have been widely used since it may resemble psoriasis human. Imiquimod effects on development, peak, and resolution of mice skin are crucial. Thus, this study aims at evaluating IMQ effects on psoriasis mice model skin tissue changes. Twenty-seven female Balb/c mice 8-11 weeks, 20-25 grams body weight (BW) were randomize equally into control (A) and experimental groups (B,C). Over 25 grams BW mice after acclimatization were excluded. The mice had shaved their back then topically applied emollient (A) and IMQ (B,C) for 7 consecutive days. On day 8 (A,B) and 15 (C), mice were terminated and back skin harvested for histopathological examination. Psoriasis Area Severity Index (PASI) and Baker’s scores were used to measure macroscopic and microscopic skin changes. All groups score differences were assessed using Kruskal Wallis then Mann Whitney tests. Skin change
s gradually appeared from day 2 to 7 of IMQ applications and faded after 2 days IMQ discontinuation. On day 7, all mice showed means of PASI scores 0(A);9,00±2,69(B);8,11±1,62(C) with significant differences between experimental to control groups (p = 0.00). Means of PASI scores from mice group C on day 15 showed similar to group A (p = 0.10). Means of Baker’s scores from all groups were 1,16±0,25(A); 3,33±1,25(B);1,39±0,22(C) and group B mice showed different significant scores from others (p = 0.00). As conclusion, the IMQ showed a reversible effect on skin tissue changes in psoriasis mice model.The abstract should summarize the contents of the paper and should contain at least 70 and at most 200 words. It should be set in 9-point font size, justified and should have a hanging indent of 2-centimenter. There should be a space before of 12-point and after of 30-point.
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