Authors:
Nini Rahmawati
1
;
Rosmayati
1
;
Delvian
2
;
Mohammad Basyuni
2
and
Hirosuke Oku
3
Affiliations:
1
Faculty of Agriculture, Universitas Sumatera Utara, Medan 20155, Sumatera Utara, Indonesia
;
2
Faculty of Forestry, Universitas Sumatera Utara, Medan20155,Sumatera Utara, Indonesia
;
3
Centers of Molecular Biosciences, University of the Ryukyus, Okinawa, Japan
Keyword(s):
Soybean, saline tolerant gene, mass selection
Abstract:
Saline soils are land that has not been utilized widely for the cultivation of soybean due to the toxic effects that interfere with the growth of the plant. This research aims to get the soybean genotypes that have salinity resistant through mass selection methods and gene expression testing of third generation of saline tolerant of the soybean genotypes that salinity resistant (F3). The research of mass selection conducted on research’s land with salinity of land 5-6 mmhos/cm and molecular analysis carried out at the Center of Molecular Biosciences University of the Ryukyus, Japan. Molecular analysis of saline tolerant gene at the root of soybean selection results F3 and soybean grobogan varieties showed mRNA expression gene DREB5, GPRP3, P5CS, bZIP, ERF and NHX1 higher in selected soybean that salinity resistant F3 that was treated by salinity compared to the controls, while the level of gene expression GmCLC1 and PAP3 lower than the control. Comparison of gene expression levels in
soybeans that given salinity stress show there has been an increased of expression genes that associated with the ability of adaptation of plants to salinity stress.
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