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Authors: Peter Sarkozy 1 ; Márton Enyedi 2 and Peter Antal 1

Affiliations: 1 Budapest University of Technology and Economics, Hungary ; 2 Hungarian Academy of Sciences, Hungary

Keyword(s): Next Generation Sequencing, Homopolymer Errors, Pyrosequencing, Semiconductor Sequencing, Visualization.

Related Ontology Subjects/Areas/Topics: Algorithms and Software Tools ; Artificial Intelligence ; Bioinformatics ; Biomedical Engineering ; Image Analysis ; Next Generation Sequencing ; Sequence Analysis ; Soft Computing ; Visualization

Abstract: We characterized the error sources of multiple resequencing measurements performed on the Ion Torrent Personal Genome Machines and the Roche 454 sequencing platforms. Homopolymer insertions and deletions are the most common error types for these platforms, and there are many underlying factors which define their occurrence patterns. In the paper we investigate the effect of flow order, specifically the difference in the average value of the flow values for each homopolymer run length, based on the position in the flow cycle.

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Paper citation in several formats:
Sarkozy, P.; Enyedi, M. and Antal, P. (2014). Flow Index based Characterization of next Generation Sequencing Errors - Visualizing Pyrosequencing and Semiconductor Sequencing to Cope with Homopolymer Errors. In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms (BIOSTEC 2014) - BIOINFORMATICS; ISBN 978-989-758-012-3; ISSN 2184-4305, SciTePress, pages 271-277. DOI: 10.5220/0004924902710277

@conference{bioinformatics14,
author={Peter Sarkozy. and Márton Enyedi. and Peter Antal.},
title={Flow Index based Characterization of next Generation Sequencing Errors - Visualizing Pyrosequencing and Semiconductor Sequencing to Cope with Homopolymer Errors},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms (BIOSTEC 2014) - BIOINFORMATICS},
year={2014},
pages={271-277},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0004924902710277},
isbn={978-989-758-012-3},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms (BIOSTEC 2014) - BIOINFORMATICS
TI - Flow Index based Characterization of next Generation Sequencing Errors - Visualizing Pyrosequencing and Semiconductor Sequencing to Cope with Homopolymer Errors
SN - 978-989-758-012-3
IS - 2184-4305
AU - Sarkozy, P.
AU - Enyedi, M.
AU - Antal, P.
PY - 2014
SP - 271
EP - 277
DO - 10.5220/0004924902710277
PB - SciTePress

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