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Authors: Soumita Ghosh 1 ; Andreas Schmidt 1 and Dieter Trau 2

Affiliations: 1 AyoxxA Living Health Technologies, Singapore ; 2 National University of Singapore, Singapore

Keyword(s): Fast Inexact Graph Matching, Microarray Bead Detection, In-situ Encoded Bead-based Array, Graph Spectra, Sequence Alignment.

Related Ontology Subjects/Areas/Topics: Applications and Services ; Computer Vision, Visualization and Computer Graphics ; Features Extraction ; Image and Video Analysis ; Image Registration ; Medical Image Applications ; Segmentation and Grouping ; Shape Representation and Matching

Abstract: This paper presents an automatic bead detection and bead array pattern matching technique developed for the In-situ Encoded Bead-based Array (IEBA) technology. A supervised learning based bead detection technique robust to irregular illumination variations and noise is developed. An efficient and effective graph matching technique that combines graph spectral analysis and sequence alignment is used to match bead array patterns. The matching algorithm proposed is rotation and scale-invariant. The pattern matching algorithm performs in-exact matching and is capable of handling very large numbers of outliers in the target graph as well as large number of occlusions in the template graph. The matching algorithm uses dynamic programming and can give good time performances dependent only on the number of nodes in the template and target graphs, irrespective of the number of outliers and occlusions. The algorithm can detect and match large number of beads in a few seconds.

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Paper citation in several formats:
Ghosh, S.; Schmidt, A. and Trau, D. (2012). FAST BEAD DETECTION AND INEXACT MICROARRAY PATTERN MATCHING FOR IN-SITU ENCODED BEAD-BASED ARRAY. In Proceedings of the International Conference on Computer Vision Theory and Applications (VISIGRAPP 2012) - Volume 1: VISAPP; ISBN 978-989-8565-04-4; ISSN 2184-4321, SciTePress, pages 5-14. DOI: 10.5220/0003826800050014

@conference{visapp12,
author={Soumita Ghosh. and Andreas Schmidt. and Dieter Trau.},
title={FAST BEAD DETECTION AND INEXACT MICROARRAY PATTERN MATCHING FOR IN-SITU ENCODED BEAD-BASED ARRAY},
booktitle={Proceedings of the International Conference on Computer Vision Theory and Applications (VISIGRAPP 2012) - Volume 1: VISAPP},
year={2012},
pages={5-14},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003826800050014},
isbn={978-989-8565-04-4},
issn={2184-4321},
}

TY - CONF

JO - Proceedings of the International Conference on Computer Vision Theory and Applications (VISIGRAPP 2012) - Volume 1: VISAPP
TI - FAST BEAD DETECTION AND INEXACT MICROARRAY PATTERN MATCHING FOR IN-SITU ENCODED BEAD-BASED ARRAY
SN - 978-989-8565-04-4
IS - 2184-4321
AU - Ghosh, S.
AU - Schmidt, A.
AU - Trau, D.
PY - 2012
SP - 5
EP - 14
DO - 10.5220/0003826800050014
PB - SciTePress

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