Authors:
S. Py
1
;
B. Wacogne
2
;
L. Pazart
1
;
A. Coaquette
1
;
W. Boireau
3
;
G. Herbein
2
and
G. Thiriez
1
Affiliations:
1
Besançon University Hospital, France
;
2
Besançon University Hospital and University of Franche-Comté, France
;
3
University of Franche-Comté, France
Keyword(s):
Cytomegalovirus, Screening, Biochips, Preterm Infants, Breastfeeding.
Related
Ontology
Subjects/Areas/Topics:
Biomedical Engineering
;
Biomedical Equipment
;
Biomedical Instrumentation
;
Biomedical Instruments and Devices
;
Biomedical Sensors
Abstract:
Cytomegalovirus (CMV) is the leading cause of neonatal viral infection and can have a significant impact
on the neurosensory development of newborns and especially preterm infants. While congenital CMV
infection affects about 2-5% of very preterm infants, the risk of postnatal infection, particularly through
breast milk, is much higher in this population (20%). However, infection could be considerably reduced by
an early and fast screening of breast milk. Indeed, a treatment (freezing or pasteurization) of contaminated
breast milk only could eliminate the virus. The idea of this position paper is that breast milk screening
would help defining an appropriate and personalized feeding strategy. We explain how to develop a CMV
biosensor to detect the virus in milk. It employs specific CMV antibodies grafted on a biochip surface to
capture viral material and additional detection antibodies in a “sandwich assay” type system. Detection is
based on optical absorption. It will be tested with
a device developed previously. However, preliminary
results obtained in ELISA technique with breast milk and homemade antibodies are presented in this
position paper. The ulterior motive of this work is the fabrication of an autonomous and automated device
that will be experimented in subsequent diagnosis strategy trial.
(More)