APPROACH TO ENABLE AN AUTOMATIC PRE-PROCESSING OF qRT-PCR - Analysis

Marco Franke; Klaus-Dieter Thoben; Rainer Söller

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Paper

APPROACH TO ENABLE AN AUTOMATIC PRE-PROCESSING OF qRT-PCR - Analysis

Topics: Algorithms and Software Tools

In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 0BIOSTEC, 281-285, 2012 , Vilamoura, Algarve, Portugal

Authors: Marco Franke ¹ ; Klaus-Dieter Thoben ¹ and Rainer Söller ²

Affiliations: ¹ University Bremen, Germany ; ² Qiagen Hamburg, Germany

Keyword(s): Polymerase Chain Reaction, Automated Pre-processing of qRT-PCR Results, Noise Removal for Sigmoid Curves.

Related Ontology Subjects/Areas/Topics: Algorithms and Software Tools ; Bioinformatics ; Biomedical Engineering

Abstract: A fully automated analysis method for real-time PCR samples can be achieved through an automated noise removal. This results in a guaranteed robustness against the factors that repeatedly impact the analysis of real-time PCR data and usually lead to a manual analysis by skilled experts. The presented approach attains a degree of robustness which allows an automated analysis for real-time PCR. This new automated pre-processing method improves the real-time PCR data for the analysis in such a way that accepted qualitative analysis methods can be used in an automated way. Furthermore, the article shows an evaluation of an implemented demonstrator for an automated analysis which combines the developed pre-processing method with the second derivation method for the qualitative analysis.

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Paper citation in several formats:

Franke, M.; Thoben, K. and Söller, R. (2012). APPROACH TO ENABLE AN AUTOMATIC PRE-PROCESSING OF qRT-PCR - Analysis. In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms (BIOSTEC 2012) - BIOINFORMATICS; ISBN 978-989-8425-90-4; ISSN 2184-4305, SciTePress, pages 281-285. DOI: 10.5220/0003734102810285

@conference{bioinformatics12,
author={Marco Franke. and Klaus{-}Dieter Thoben. and Rainer Söller.},
title={APPROACH TO ENABLE AN AUTOMATIC PRE-PROCESSING OF qRT-PCR - Analysis},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms (BIOSTEC 2012) - BIOINFORMATICS},
year={2012},
pages={281-285},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003734102810285},
isbn={978-989-8425-90-4},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms (BIOSTEC 2012) - BIOINFORMATICS
TI - APPROACH TO ENABLE AN AUTOMATIC PRE-PROCESSING OF qRT-PCR - Analysis
SN - 978-989-8425-90-4
IS - 2184-4305
AU - Franke, M.
AU - Thoben, K.
AU - Söller, R.
PY - 2012
SP - 281
EP - 285
DO - 10.5220/0003734102810285
PB - SciTePress