Authors:
Simon Martin Manyanza Nzilibili
1
;
Muh. Abduh Dwi Putra
2
and
Ahmad Yudianto
3
Affiliations:
1
Forensic Science Program, Post Graduate School, Airlangga University, 4-6 Airlangga Rd., 60286, Surabaya - Indonesia., Ministry of Health, Community Development, Gender Elderly and Children and Dodoma – Tanzania., Tanzania
;
2
Forensics and Medico-legal Department, Faculty of Medicine, Airlangga University and Surabaya – Indonesia, Indonesia
;
3
Forensic Science Program, Post Graduate School, Airlangga University, 4-6 Airlangga Rd., 60286, Surabaya - Indonesia., Forensics and Medico-legal Department, Faculty of Medicine, Airlangga University and Surabaya – Indonesia, Indonesia
Keyword(s):
Ethyl, Spectacle, Trace Evidence, 70% Ethylated swab
Abstract:
Trace evidence recovery has been studied to involve a number of methods with the inclusion of swabbing methods. In swabbing, different fluids have also been proposed with minimal highlight to ethylated swabbing (prepared from ethanol). Through the use of swabbing fluid at 70% dilution (70% ethylated swab) run simultaneously with distilled water swab, the findings discovered were that 70% ethylated swab was found to concentrate large amount of DNA sample (1421µg/ml), twice the amount recovered by distilled water swab (654.5µg/ml). In terms of purity of recovered DNA; 70% ethylated swab presented nearly similar purity with distilled water DNA pellet (ethylated swab yielded 1.219 purity ratio while distilled water swab yielded 1.176). Electrophoresis DNA molecule migration displayed multiple bands in contrast with 70% ethylated swab from positive to negative electrode. This shows the presence of both small and larger sized DNA fragments. Distilled water swab displayed one deep band near
negative terminal electrode. Thereby; the study finding analysis illustrates and suggests that 70% ethylated swab is a useful and strong method in recovering trace DNA evidences for successful DNA profile establishment. In addition, spectacle is prospective a potential harbor of trace DNA sample for forensic investigation.
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