Authors:
R. G. Silva
;
J. S. Silva
;
A. A. Vicente
;
J. A. Teixeira
and
R. C. Martins
Affiliation:
Universidade do Minho, Portugal
Keyword(s):
UV-VIS-SWNIR spectroscopy, Real-time monitoring, Multivariate calibrarion.
Related
Ontology
Subjects/Areas/Topics:
Artificial Intelligence
;
Bioinformatics
;
Biomedical Engineering
;
Biomedical Signal Processing
;
Data Manipulation
;
Health Engineering and Technology Applications
;
Human-Computer Interaction
;
Laser and Opto-Electronics Signal Processing Applications
;
Methodologies and Methods
;
Neurocomputing
;
Neurotechnology, Electronics and Informatics
;
Pattern Recognition
;
Physiological Computing Systems
;
Real-Time Systems
;
Sensor Networks
;
Soft Computing
Abstract:
One of the most studied bioprocesses is fermentation by yeasts. Although this is true, there is still the lack of real-time instrumentation that is capable of providing detailed information on metabolic state of fermentations. In this research we explore the possibility of using UV-VIS-SWNIR spectroscopy as a high-output, non-destructive and multivariate methodology of monitoring beer fermentation. We herein report the implementation of the a fibber optics sensor and the capacity for detecting key parameters by partial least squares regression for biomass, extract, pH and total sugars. Results show that UV-VIS-SWNIR is a robust technique for monitoring beer fermentations, being able to provide detailed information spectroscopic fingerprinting of the process. Calibrations were possible to obtain for all the studied parameters with R2 of 0.85 to 0.94 in the UV-VIS region and 0.95 to 0.97 in the VIS-SWNIR region. This preliminary study allowed to conclude that further improvements in ex
perimental methodology and signal processing may turn this technique into a valuable instrument for detailed metabolic studies in biotechnology.
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