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Authors: R. G. Silva ; J. S. Silva ; A. A. Vicente ; J. A. Teixeira and R. C. Martins

Affiliation: Universidade do Minho, Portugal

ISBN: 978-989-8111-65-4

Keyword(s): UV-VIS-SWNIR spectroscopy, Real-time monitoring, Multivariate calibrarion.

Related Ontology Subjects/Areas/Topics: Artificial Intelligence ; Bioinformatics ; Biomedical Engineering ; Biomedical Signal Processing ; Data Manipulation ; Health Engineering and Technology Applications ; Human-Computer Interaction ; Laser and Opto-Electronics Signal Processing Applications ; Methodologies and Methods ; Neurocomputing ; Neurotechnology, Electronics and Informatics ; Pattern Recognition ; Physiological Computing Systems ; Real-Time Systems ; Sensor Networks ; Soft Computing

Abstract: One of the most studied bioprocesses is fermentation by yeasts. Although this is true, there is still the lack of real-time instrumentation that is capable of providing detailed information on metabolic state of fermentations. In this research we explore the possibility of using UV-VIS-SWNIR spectroscopy as a high-output, non-destructive and multivariate methodology of monitoring beer fermentation. We herein report the implementation of the a fibber optics sensor and the capacity for detecting key parameters by partial least squares regression for biomass, extract, pH and total sugars. Results show that UV-VIS-SWNIR is a robust technique for monitoring beer fermentations, being able to provide detailed information spectroscopic fingerprinting of the process. Calibrations were possible to obtain for all the studied parameters with R2 of 0.85 to 0.94 in the UV-VIS region and 0.95 to 0.97 in the VIS-SWNIR region. This preliminary study allowed to conclude that further improvements in exp erimental methodology and signal processing may turn this technique into a valuable instrument for detailed metabolic studies in biotechnology. (More)

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Paper citation in several formats:
G. Silva R.; S. Silva J.; A. Vicente A.; A. Teixeira J.; C. Martins R. and (2009). IN-SITU, REAL-TIME BIOREACTOR MONITORING BY FIBER OPTICS SENSORS.In Proceedings of the International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2009) ISBN 978-989-8111-65-4, pages 327-336. DOI: 10.5220/0001549503270336

@conference{biosignals09,
author={R. {G. Silva} and J. {S. Silva} and A. {A. Vicente} and J. {A. Teixeira} and R. {C. Martins}},
title={IN-SITU, REAL-TIME BIOREACTOR MONITORING BY FIBER OPTICS SENSORS},
booktitle={Proceedings of the International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2009)},
year={2009},
pages={327-336},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001549503270336},
isbn={978-989-8111-65-4},
}

TY - CONF

JO - Proceedings of the International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2009)
TI - IN-SITU, REAL-TIME BIOREACTOR MONITORING BY FIBER OPTICS SENSORS
SN - 978-989-8111-65-4
AU - G. Silva, R.
AU - S. Silva, J.
AU - A. Vicente, A.
AU - A. Teixeira, J.
AU - C. Martins, R.
PY - 2009
SP - 327
EP - 336
DO - 10.5220/0001549503270336

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