Authors:
A. Martínez-Olmos
;
A. J. Palma
;
J. Ballesta-Claver
;
M. C. Valencia-Miron
and
L. F. Capitan-Vallvey
Affiliation:
University of Granada, Spain
Keyword(s):
Electrochemiluminescence, Portable instrumentation, Potentiostat, Biosensor, Screen-printed device, Lactate determination, Saliva.
Related
Ontology
Subjects/Areas/Topics:
Biomedical Engineering
;
Biomedical Instrumentation
;
Biomedical Instruments and Devices
;
Biomedical Sensors
;
Low-Power Design
Abstract:
A new hand-held luminometer for electrochemiluminescence (ECL)-based one-shot biosensor for lactate is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of luminol, all reagents immobilized in a Methocel membrane. The measurement of ECL from a screenprinted electrode by a portable instrument designed and developed by the authors makes it possible to determine lactate concentration. The compositions of the membrane and reaction conditions have been optimized to obtain adequate sensitivity. The one-shot biosensor responds to lactate rapidly, with the typical ECL acquisition time being 3 min, with a linearized dependence whose dynamic range was from 9·10-6 to 2·10-3 M, a detection limit of 2.4·10-6 M and a sensor-to-sensor reproducibility (relative standard deviation RSD) around 10 % at the medium level of the range. The performance of the ECL one-shot biosensor and portable instrument was tested for the non invasive analysis of l
actate in saliva, validating the results against a reference procedure.
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