Authors:
Mohammad Basyuni
1
;
Irma Deni
1
;
Bejo Slamet
1
;
Yuntha Bimantara
1
;
Rahmah Hayati
1
;
Rizka Amelia
1
;
Hirosuke Oku
2
and
Hiroshi Sagami
3
Affiliations:
1
Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatera 20155, Indonesia
;
2
Molecular Biotechnology Group, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan
;
3
Institute of Multidisciplinary Research for Advanced Material, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan
Keyword(s):
chemotaxonomic marker, dolichol, dehydrodolichol, pine
Abstract:
This current work examines the polyisoprenoids (dehydrodolichol or polyprenol and dolichol) profiling and conformation from Norfolk Island pine Araucaria heterophylla (Araucariaceae) and Australian pine Casuarina equisetifolia (Casuarinaceae). The pattern and structure of polyisoprenes were determined by two-plate thin layer chromatography (2P-TLC). The polyisoprene pattern in the leaves was found and categorized into two categories. Group-I, showing a majority of dolichols over dehydrodolichols was detected in A. heterophylla. These dolichols showed as one longer dolichol tribe (C40–C110). Group-II, exhibiting the incidence of the pair polyprenols and dolichols, was traced in C. equisetifolia. Dolichol concentrations were faintly extra richness detected comparing to dehydrodolichols (approximately 54%:46%) in this species. Dolichols with chain length of C75–C95 and shorter dehydrodolichol (C50–C55) were detected in C. equisetifolia. This study suggested that different pattern of fic
aprenols, shorter-chain and longer dolichols are modulated in both pine species.
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