Authors:
You-Lin Wu
1
;
Po-Yen Hsu
1
;
Chung-Ping Hsu
2
and
Wen- Cheng Liu
1
Affiliations:
1
National Chi Nan University, Taiwan
;
2
Taichung Veterans General Hospital, Taiwan
Keyword(s):
DNA bases detection, Polysilicon wire sensor, Label-free.
Related
Ontology
Subjects/Areas/Topics:
Biomedical Engineering
;
Biomedical Instruments and Devices
;
Biomedical Sensors
Abstract:
In this paper we report the electrical measurement of sequence-specific and label-free deoxyribonucleic acid (DNA) by using poly-silicon (poly-Si) wire. Four single-strained (ss) DNA bases, adenine (A), thymine (T), cytosine (C) and guanines (G), as well as double-stranded (ds) DNA sequences, ds(A-T) and ds(C-G), with different lengths and concentrations were dropped onto the poly-Si wire surface, and currents flowing through the poly-Si wire channel were determined. It is found that the amount change of the channel current I for ssDNAs with fixed length and fixed concentration is that T > C > G > A. For dsDNA sequence, we observe that ds(A-T) has higher I than does ds(C-G). We also prove that single base change in ssDNA is feasible by using the poly-Si wire sensor.