Authors:
Hmida Rojbani
1
and
Atef Hamouda
2
Affiliations:
1
University of Strasbourg and University Tunis El Manar, France
;
2
University Tunis El Manar, Tunisia
Keyword(s):
Electron Tomography, Resolution, Metric, 3D Structures, Tilt Angles.
Related
Ontology
Subjects/Areas/Topics:
Applications and Services
;
Computer Vision, Visualization and Computer Graphics
;
Image and Video Analysis
;
Image Formation and Preprocessing
;
Image Formation, Acquisition Devices and Sensors
;
Medical Image Applications
;
Shape Representation and Matching
;
Visual Attention and Image Saliency
Abstract:
Motivate by reaching a better understanding of the biological cells, scientists use the Transmission Electron
Microscope (TEM) to investigate their inner structures. The cryo-electron tomography (cryo-ET) offers the
possibility to reconstruct 3D structure reconstruction of a cell slice, that by tilting it according different angles.
The resolution limits is the biggest challenge in the cryo-ET. The two phases involved in increasing the
resolution are the acquisition phase and the reconstruction phase. In this work, we focus in the last one, as the
biologists treat the acquisition phase within the phase of acquisition itself. The resolution of reconstruction
depends on many factors such as: (1) the noisy and missing information from the collected projections data,
(2) the capacity of processing large data sets, (3) the parametrization of the contrast function of the microscope,
(4) errors of the tilt angles used in projections. In this paper, we presented a new method to evaluate
the
resolution of a reconstruction algorithm. Then the proposed method is used to show the relation between
errors of the tilt angles used in projection and the degradation of the resolution. The resolution evaluation tests
are made with different reconstruction methods (analytic and algebraic) applied on synthetic and real data.
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