Authors:
James F. Rusling
1
;
Colleen Krause
2
;
Brunah Otieno
2
;
Karteek Kadimisetty
2
;
Chi Tang
2
;
Abhay Vaze
2
and
Gregory Bishop
2
Affiliations:
1
University of Connecticut, University of Connecticut Health Center and National University of Ireland, United States
;
2
University of Connecticut, United States
Keyword(s):
Cancer, Biomarkers, Multiplex, Protein Detection, Microfluidics, Nanoparticles.
Related
Ontology
Subjects/Areas/Topics:
Biomedical Engineering
;
Biomedical Instrumentation
;
Biomedical Instruments and Devices
;
Biomedical Sensors
;
Emerging Technologies
;
Microelectronics
;
Nanotechnologies
;
Telecommunications
;
Wireless and Mobile Technologies
;
Wireless Information Networks and Systems
Abstract:
Accurate, sensitive, multiplexed detection of biomarker proteins in serum and tissue holds significant promise for personalized cancer diagnostics and therapy monitoring. Here we describe fabrication details of a modular microfluidic system featuring a small chamber for on-line protein capture from serum by magnetic beads, positioned upstream of a nanostructured multi-sensor array chamber to achieve high sensitivity for up to eight proteins, with the ability to expand to many more proteins. Microfluidic chambers are made by templating PDMS channels on machined aluminum molds to avoid lithography, and mounted in hard plastic housings equipped with inlet and outlet lines and interfaced with valves. Gold immunoarrays fabricated by screen or ink-jet printing, or wet chemical etching of gold films utilize amperometry or electrochemiluminescence (ECL) detection. These arrays are interfaced with microfluidics to achieve well-controlled mass transport leading to excellent signal/noise and un
precedented sensitivities. With interest in low cost point of care (POC) systems, we developed a module to also facilitate automated microfluidic reagent and sample delivery utilizing an open source microcontroller and micropumps, with ECL detection by camera.
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