Authors:
Bruno Wacogne
1
;
2
;
Céline Codjiova
2
;
Jovanne Palvair
2
;
Naïs Vaccari
2
;
Mélanie Couturier
3
;
Alain Rouleau
2
and
Annie Frelet-Barrand
2
Affiliations:
1
Centre Hospitalier Universitaire de Besançon, Centre d’Investigation Clinique, INSERM CIC 1431, 25000, Besançon, France
;
2
Université de Franche-Comté, CNRS, institut FEMTO-ST, F-25000 Besançon, France
;
3
MedInnPharma, 4 rue Charles Bried, 25000 Besançon France
Keyword(s):
White Light Spectroscopy, Mammalian Cells, Viability, Car T-Cells.
Abstract:
CAR T-cells are highly promising medical products for personalized medicine, but their long production is complex and require extensive quality controls, which result in prohibitive costs for most patients. These controls include monitoring of cell concentration, viability, and possible contamination detection. To simplify CAR T production, these controls should ideally be conducted online in a closed system, without sampling from the bioreactor. Recently, we proposed white light spectroscopy as a method for online monitoring of cell concentration. In this paper, we demonstrate that this optical method can also assess cell viability. We define a cell suspension "quality value" which shows a linear relationship with cell viability estimated by conventional methods. This relationship varies depending on the techniques used and the dominant T-cell death process induced. We then hypothesise that the quality score could serve as a general indicator of cell suspension health, as it is not
dependent on any biophysical-chemical interaction or instrument. Overall, the correlation between conventional and optical methods, together with previously published results on cell concentration monitoring, suggests that white light spectroscopy is a promising on-line and sample-free option for monitoring CAR T production.
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