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Authors: I. Gurevich 1 ; I. Koryabkina 1 ; E. Kozina 2 ; A. Myagkov 3 ; H. Niemann 4 ; M. Ugrumov 2 and V. Yashina 1

Affiliations: 1 Dorodnicyn Computing Centre of the Russian Academy of Sciences, Russian Federation ; 2 Koltzov Institute of Developmental Biology of the Russian Academy of Sciences, Russian Federation ; 3 Lomonosov Moscow State University, Russian Federation ; 4 Friedrich-Alexander University of Erlangen-Nuremberg, Germany

Abstract: This work is devoted to the description of an experimental data acquisition automated method which is required to fill the model of Parkinson’s disease preclinical stage. Digital images of the immunostained brain sections of experimental animals are used as a data source. Proposed method: 1) is based on following mathematical morphology operations: opening, grayscale reconstruction, closing, bot-hat transformation, morphological gradient, watershed transformation; 2) enables: to smooth heterogeneous complex background; to select small objects on images depended on given sizes and gray values; to eliminate out-of-focus objects; to separate close objects; to calculate features of selected objects; 3) is intended for automatic extraction of dopaminergic neurons terminals on striatum frontal section images. Experimental investigations confirmed possibility and suitability of section images automated processing and analysis by means of the method. The results of the method use are segmen ted object contours binary image and object feature list. (More)

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Paper citation in several formats:
Gurevich, I.; Koryabkina, I.; Kozina, E.; Myagkov, A.; Niemann, H.; Ugrumov, M. and Yashina, V. (2009). A Method for Automatic Extraction of Dopaminergic Neuron Terminals on Striatum Frontal Section Images. In Proceedings of the 2nd International Workshop on Image Mining Theory and Applications (VISIGRAPP 2009) - Workshop IMTA; ISBN 978-989-8111-80-7, SciTePress, pages 30-39. DOI: 10.5220/0001964600300039

@conference{workshop imta09,
author={I. Gurevich. and I. Koryabkina. and E. Kozina. and A. Myagkov. and H. Niemann. and M. Ugrumov. and V. Yashina.},
title={A Method for Automatic Extraction of Dopaminergic Neuron Terminals on Striatum Frontal Section Images},
booktitle={Proceedings of the 2nd International Workshop on Image Mining Theory and Applications (VISIGRAPP 2009) - Workshop IMTA},
year={2009},
pages={30-39},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001964600300039},
isbn={978-989-8111-80-7},
}

TY - CONF

JO - Proceedings of the 2nd International Workshop on Image Mining Theory and Applications (VISIGRAPP 2009) - Workshop IMTA
TI - A Method for Automatic Extraction of Dopaminergic Neuron Terminals on Striatum Frontal Section Images
SN - 978-989-8111-80-7
AU - Gurevich, I.
AU - Koryabkina, I.
AU - Kozina, E.
AU - Myagkov, A.
AU - Niemann, H.
AU - Ugrumov, M.
AU - Yashina, V.
PY - 2009
SP - 30
EP - 39
DO - 10.5220/0001964600300039
PB - SciTePress

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