Authors:
Rifqi Syahbana
1
;
Emma Zaidar Nasution
2
and
Rumondang Bulan Nasution
2
Affiliations:
1
Postgraduate Chemistry Study Programme, Universitas Sumatera Utara, Medan, Indonesia
;
2
Department of Chemistry, Universitas Sumatera Utara, Jl. Bioteknologi No. 1, Medan
Keyword(s):
Bioethanol, Immobilization, Inoculum volume, Saba Banana Hump, Zymomonas Mobilis.
Abstract:
Bioethanol is a liquid produced from the sugar fermentation process from carbohydrate sources by using the microorganism. In present study, Saba banana hump (Musa paradisiacal. L) sample was isolated by precipitating the starch with water for 12 hours. Furthermore, the starch was hydrolyzed using HCl 25%, and 14.01% of glucose solution was obtained. The starch obtained was tested using Fourier-transform infrared spectroscopy. Then the sample was fermented using Zymomonas mobilis microorganism immobilized by Ca-Alginate 4% with variation of inoculums 5%, 10%, 15% (v/v). Bioethanol from fermentation process was tested using gas chromatography (GC). The highest bioethanol content is 79.01% and the highest productivity that obtain was 2.66 g/L.Hours.
1 INTRODUCTION
Banana hump is the bottom of a banana plant that has characteristics that are not too hard, banana hump waste has a starch composition of approximately 70%, 20% water, the rest is protein and vitamins. For the banana hump it
self has not been used optimally, where the banana weevil waste is sometimes only as animal feed or left alone to rot. The carbohydrate content of banana hump is very potential as a source of bioethanol, so that it can increase the use value of the banana weevil and can optimize the utilization of the banana hump. As for one type of carbohydrate that is in the banana weevil is starch.
Starch is a carbohydrate polymer consisting of glucose monomers
(More)