Authors:
Ahmad Anwar Zainuddin
1
;
2
;
Mohd Afiq Mohd Asri
1
;
Cyril Guines
3
;
Muhammad Zahid Zabedi
4
;
Khin Maung Htay
1
;
Abdul Hakim Ab Rahim
1
;
Matthieu Chatras
4
;
Arnaud Pothier
4
and
Anis Nurashikin Nordin
1
Affiliations:
1
Kulliyyah of Engineering, International Islamic University Malaysia, 53100 Gombak, Kuala Lumpur, Malaysia
;
2
Department of Computer Science and Engineering, School of Engineering, Manipal International University, 71800 Nilai, Negeri Sembilan, Malaysia
;
3
XLIM-UMR 7252, University of Limoges/CNRS, 87060 Limoges, France 43D Synapsis, 44a, Jalan Keluli AK7/AK, Taman Perindustrian Bukit Raja Selatan, 40000 Shah Alam, Selangor, Malaysia
;
4
Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linkoping University, 58183 Linkoping, Sweden
Keyword(s):
Dengue, Biosensor, Sensor Arrays, Integrated Electrochemical-Quartz Crystal Microbalance, Point-of-Care Diagnostics.
Abstract:
This paper presents the current progress towards a lab-on-chip biosensor for early dengue detection, consisting of an integrated sensor with dual-function working electrode that enables in-situ measurements of both electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) enclosed in a miniaturized 3D-printed package equipped with electrical contacts and sample fluid delivery to the quartz biosensor array. The sensors consist of an array of three 10 MHz IEQCM biosensors on a single quartz substrate. Early validation is performed for future dengue sensing application. We report the design, optimisation, and fabrication of the sensors, as well as early optimisation and validation of surface bioconjugation of antibodies. This lab-on-chip has the potential to provide accurate dengue detection due to its high sensitivity and dynamic range, as well as providing rapid and early dengue detection in point-of-care settings.