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Authors: Muhammad Taufik 1 ; Desi Ardilla 2 ; Mariany Razali 3 ; Endang Susilawati 4 ;  Afniwati 4 and Nurul Fadillah 1

Affiliations: 1 Chemistry Department, Faculty of Mathematic and Natural Science, Universitas Sumatera Utara, Medan 20155, Sumatera Utara, Indonesia, Indonesia ; 2 Agricultural Technology Department, Universitas Muhammadiyah Sumatera Utara, Medan, Indonesia, Indonesia ; 3 Pharmacy Department, Universitas Tjut Nyak Dhien, Medan, Indonesia, Indonesia ; 4 Nurse Department, Poltekkes Kemenkes Medan, Indonesia

Keyword(s): Saliva, FTIR, Nicotine, Uv-Visible Spectrophotometer, Extraction

Abstract: An accurate and simple method of extraction and analysis needs to be developed in the context of investigating nicotine in the saliva of active smokers. This study aims to prepare, extract and analyse nicotine from the saliva of active smokers. The preparation process is carried out from the location where the samples were taken. Extraction was carried out using sonication coupling maceration for 15 minutes. Qualitative analysis used spot test with Cyanogen bromide reagent and quantitative analysis used UV spectrophotometer. Ultra Violet (UV) spectrophotometer analysis at the optimum wavelength of 260 nm resulted in a sample concentration of Saliva A = 1.4 ppm and Saliva B = 1.6 ppm

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Paper citation in several formats:
Taufik, M.; Ardilla, D.; Razali, M.; Susilawati, E.; Afniwati. and Fadillah, N. (2022). Extraction and Analysis of Nicotine from the Saliva of Active Smokers using UV Spectroscopy. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - IMC-SciMath; ISBN 978-989-758-556-2, SciTePress, pages 616-620. DOI: 10.5220/0010614700002775

@conference{imc-scimath22,
author={Muhammad Taufik. and Desi Ardilla. and Mariany Razali. and Endang Susilawati. and Afniwati. and Nurul Fadillah.},
title={Extraction and Analysis of Nicotine from the Saliva of Active Smokers using UV Spectroscopy},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - IMC-SciMath},
year={2022},
pages={616-620},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010614700002775},
isbn={978-989-758-556-2},
}

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - IMC-SciMath
TI - Extraction and Analysis of Nicotine from the Saliva of Active Smokers using UV Spectroscopy
SN - 978-989-758-556-2
AU - Taufik, M.
AU - Ardilla, D.
AU - Razali, M.
AU - Susilawati, E.
AU - Afniwati.
AU - Fadillah, N.
PY - 2022
SP - 616
EP - 620
DO - 10.5220/0010614700002775
PB - SciTePress

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