Authors:
Linda Margata
1
;
Jansen Silalahi
2
;
Urip Harahap
3
;
Dwi Suryanto
4
and
Denny Satria
2
Affiliations:
1
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia, Indonesia
;
2
Universitas Sumatera Utara
;
3
Faculty of Pharmacy, Universitas Sumatera Utara, Tri Dharma Street,5 Campus USU, Medan, Indonesia
;
4
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
Keyword(s):
Virgin coconut oil, Enzymatic hydrolysis, Blood glucose, Haemoglobin A1c, Superoxide dismutase
Abstract:
The aim of this study was to examine the antidiabetic and antioxidant effect of enzymatically hydrolyzed
virgin coconut oil (HVCO) in streptozotocin (STZ) induced rats. VCO was hydrolyzed enzymatically using
lipase from Rhizomucor miehei (active on sn-1,3 position). Thirty male rats were induced with 40 mg/kg
body weight (BW) STZ. Rats with blood glucose level ≥ 250 mg/dl were divided into six groups which
were given with sodium carboxymethylcellulose (CMC Na) 0.5%, metformin 45 mg/kgBW, VCO (4 and 6
ml/kgBW) and HVCO (4 and 6 ml/kgBW). Blood glucose, Haemoglobin A1c (HbA1c), superoxide
dismutase (SOD), soluble receptor advanced glycosylation end-product (sRAGE) levels, and
immunihistochemistry assay on pancreas were analyzed after 30 days of treatment. It is shown that HVCO 4
ml/kgBW and metformin were not significantly different in lowering blood glucose level. Blood glucose
level in groups treated with HVCO 4 ml/kgBW and metformin were 409.2 and 364.40 mg/dl. HVCO also
lowered HbA
1c and sRAGE levels (55.50 ng/ml and 148.40 pg/ml, respectively), while increased SOD
level (76.96 pg/ml). Insulin expression of rats treated with 4 ml/kgBW HVCO and metformin also did not
differ significantly which were 11.40% and 11.70%, respectively. HVCO exerted higher antidiabetic and
antioxidant effects that VCO did.
(More)