Authors:
Ioana R. Ivascu
1
;
Rabah Zeggari
2
;
Christian Pieralli
2
;
Bruno Wacogne
2
and
Christophe Roux
3
Affiliations:
1
Applied Sciences Faculty, Bucarest “Politehnica” University, Romania
;
2
Institut FEMTO-ST, UMR CNRS 6174, France
;
3
Service de Génétique Histologie Biologie du Développement et de la Reproduction UMR645 – Interactions Hôte-Greffon et Ingénierie Cellulaire et Génique & IFR 133: IBCT, CHU St. Jacques Université de Franche-Comté, France
Keyword(s):
In Vitro Fertilization, Maturation estimation, Optical microsystems, Refractive index measurement.
Related
Ontology
Subjects/Areas/Topics:
Biomedical Engineering
;
Biomedical Instrumentation
;
Biomedical Instruments and Devices
;
Biomedical Metrology
;
Biomedical Sensors
;
Emerging Technologies
;
Telecommunications
;
Wireless and Mobile Technologies
;
Wireless Information Networks and Systems
Abstract:
In this paper, we present an optical microsystem used to measure the refractive index of oocytes. The technique relies on oocytes refraction properties and on the optical coupling between optical fibers. A theoretical model of the system is established by means of matrix optics. A numerical fitting allows calculating the refractive index of the oocytes. In a first time we considered the oocytes like homogeneous spheres. In a second time, we considered that the oocytes consist of a shell (the zona pellucida) and a core (the cell). Experimental results showed that the refractive index of the oocytes increases during the maturation process. In this position paper, we also discuss the temporal evolution of the oocytes maturity and we point out that the maturation should not only be estimated visually but in a more continuous manner. The device we propose could be a solution for such non invasive measurements.