Authors:
T. H. Huang
1
;
Q. S. Shi
2
;
K. Wei
1
;
J. P. Gui
2
and
L. S. Zheng
2
Affiliations:
1
China Tobacco Guangxi Industrial Co. LTD., China
;
2
School of Biological Science and Medical Engineering, Beihang University, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering and Beihang University, China
Keyword(s):
reconstituted tobacco; composite enzyme; harmful components
Abstract:
To explore the influence of the composite enzymes on harmful components in reconstituted tobacco, we developed compound enzymes which included power of cellulose (23-41%, 100,000U/g), xylanase (10-23%, 100,000U/g), complex protease (41-57%, 100,000U/g), pectinase (5-6%, 100,000U/g) and amylase (1.5-3.8%, 10,000U/g). The compound enzymes were added to the reconstituted tobacco (0.4% enzymes, incubated for 1 h at 45 ± 5°C). We evaluated the quality of the reconstituted tobacco using physical and chemical indices. The results show that protein and pectin contents were effectively reduced, with improved taste of the cigarette smoke, and harmful ingredients were reduced, such as ammonia and hydrogen cyanide, in the mainstream smoke of the composite enzyme treatment group compared with the untreated control group. This approach will reduce the danger of mainstream smoke in reconstituted tobacco.