Authors:
Thomas Windbacher
1
;
Viktor Sverdlov
1
;
Siegfried Selberherr
1
;
Clemens Heitzinger
2
;
Norbert Mauser
2
and
Christian Ringhofer
3
Affiliations:
1
TU Wien, Austria
;
2
University of Vienna, Austria
;
3
Arizona State University, United States
Keyword(s):
BioFET, Field-effect biosensor, Biotin-streptavidin, Simulation, Multi-scale problem, Interface conditions.
Related
Ontology
Subjects/Areas/Topics:
Biocomputing and Biochips
;
Biomedical Engineering
;
Biomedical Instruments and Devices
;
Biomedical Sensors
;
Electrical Bio-Impedance
Abstract:
In this work the properties of a biotin-streptavidin BioFET have been studied numerically with homogenized boundary interface conditions as the link between the oxide of the FET and the analyte which contains the biosample. The biotin-streptavidin reaction pair is used in purification and detection of various biomolecules; the strong streptavidin-biotin bond can also be used to attach biomolecules to one another or onto a solid support. Thus this reaction pair in combination with a FET as the transducer is a powerful setup enabling the detection of a wide variety of molecules with many advantages that stem from the FET, like no labeling, no need of expensive read-out devices, the possibility to put the signal amplification and analysis on the same chip, and outdoor usage without the necessity of a lab.