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Authors: J. Laforêt 1 ; M. Frénéa-Robin 2 ; H. Cérémonie 1 ; F. Buret 1 and L. Nicolas 1

Affiliations: 1 AMPERE, UMR CNRS 5005, Ecole Centrale de Lyon, France ; 2 Université de Lyon; AMPERE Villeurbanne, France

Keyword(s): Dielectrophoresis, electrorotation, yeast cells, yeast protoplasts.

Related Ontology Subjects/Areas/Topics: Bio-Electromagnetism ; Biomedical Engineering ; Biomedical Instruments and Devices

Abstract: This paper is about the development of a new automated platform dedicated to cell manipulation and characterization by dielectrophoretic methods. We illustrate its possibilities by studying yeast protoplasts and yeast cells electrorotation spectra, obtained using polynomial microelectrode structures powered by computer-controlled generators. Measurements were made over the frequency range 100 kHz to 80MHz, mostly in a suspending medium of conductivity 50 mS/m inside the rotation chamber. The rotation rate of yeast protoplasts was inferior to that of whole yeast cells. To understand such behavioral differences, yeast protoplasts were modelled as single-shell spheres in a first approach.

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Paper citation in several formats:
Laforêt, J.; Frénéa-Robin, M.; Cérémonie, H.; Buret, F. and Nicolas, L. (2008). AUTOMATED CELL CHARACTERIZATION PLATFORM: APPLICATION TO YEAST PROTOPLAST STUDY BY ELECTROROTATION. In Proceedings of the First International Conference on Biomedical Electronics and Devices (BIOSTEC 2008) - Volume 1: BIODEVICES; ISBN 978-989-8111- 17-3; ISSN 2184-4305, SciTePress, pages 190-193. DOI: 10.5220/0001053401900193

@conference{biodevices08,
author={J. Laforêt. and M. Frénéa{-}Robin. and H. Cérémonie. and F. Buret. and L. Nicolas.},
title={AUTOMATED CELL CHARACTERIZATION PLATFORM: APPLICATION TO YEAST PROTOPLAST STUDY BY ELECTROROTATION},
booktitle={Proceedings of the First International Conference on Biomedical Electronics and Devices (BIOSTEC 2008) - Volume 1: BIODEVICES},
year={2008},
pages={190-193},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001053401900193},
isbn={978-989-8111- 17-3},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the First International Conference on Biomedical Electronics and Devices (BIOSTEC 2008) - Volume 1: BIODEVICES
TI - AUTOMATED CELL CHARACTERIZATION PLATFORM: APPLICATION TO YEAST PROTOPLAST STUDY BY ELECTROROTATION
SN - 978-989-8111- 17-3
IS - 2184-4305
AU - Laforêt, J.
AU - Frénéa-Robin, M.
AU - Cérémonie, H.
AU - Buret, F.
AU - Nicolas, L.
PY - 2008
SP - 190
EP - 193
DO - 10.5220/0001053401900193
PB - SciTePress

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