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Authors: Constantin Apetrei 1 ; Jose Antonio de Saja 2 and Maria Luz Rodriguez-Mendez 2

Affiliations: 1 “Dunărea de Jos” University of Galaţi, Romania ; 2 University of Valladolid, Spain

Keyword(s): Biosensor, Langmuir-Blodgett, Carbon paste electrode, Bisphthalocyanine, Tyrosinase.

Related Ontology Subjects/Areas/Topics: Biomaterials ; Biomedical Engineering ; Biomedical Instruments and Devices ; Biomedical Sensors ; Nanotechnologies

Abstract: The biosensing properties of tyrosinase biosensors were investigated for two different immobilization matrixes: carbon paste and Langmuir-Blodgett thin film. In both cases the electron mediator was the lutetium (III) bisphthalocyaninate. The electrochemical responses of biosensors towards phenol and catechol were analyzed and compared. The tyrosinase maintains its bioactivity well within the immobilization matrices. A clearly defined reduction current proportional to the phenolic compounds concentration was observed in cyclic voltammetry, which attributed to the reduction of enzymatically produced quinone at the electrode surface. It was demonstrated that the biosensor based on Langmuir-Blodgett thin film shows the best performances in terms of kinetics and detection limit for the phenolic compounds analyzed.

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Paper citation in several formats:
Apetrei, C.; de Saja, J. and Rodriguez-Mendez, M. (2012). NANOSTRUCTURED VS. CARBONACEOUS BIOSENSORS - Comparative Studies for Detection of Phenolic Compounds. In Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2012) - BIODEVICES; ISBN 978-989-8425-91-1; ISSN 2184-4305, SciTePress, pages 104-109. DOI: 10.5220/0003716701040109

@conference{biodevices12,
author={Constantin Apetrei. and Jose Antonio {de Saja}. and Maria Luz Rodriguez{-}Mendez.},
title={NANOSTRUCTURED VS. CARBONACEOUS BIOSENSORS - Comparative Studies for Detection of Phenolic Compounds},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2012) - BIODEVICES},
year={2012},
pages={104-109},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003716701040109},
isbn={978-989-8425-91-1},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2012) - BIODEVICES
TI - NANOSTRUCTURED VS. CARBONACEOUS BIOSENSORS - Comparative Studies for Detection of Phenolic Compounds
SN - 978-989-8425-91-1
IS - 2184-4305
AU - Apetrei, C.
AU - de Saja, J.
AU - Rodriguez-Mendez, M.
PY - 2012
SP - 104
EP - 109
DO - 10.5220/0003716701040109
PB - SciTePress

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