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Authors: Zhenhao Ding ; Hiromichi Kasahara ; Michihiko Nakano and Junya Suehiro

Affiliation: Kyushu University, Japan

Keyword(s): DNA Labelling, Dielectrophoresis, Rapid DNA Detection, Crossover Frequency.

Related Ontology Subjects/Areas/Topics: Biomedical Engineering ; Biomedical Instruments and Devices ; Biomedical Sensors ; Electrical Bio-Impedance ; Microelectronics

Abstract: Polymerase chain reaction (PCR) is one of the most sensitive and specific detection methods of bacterial and viral infections. The authors proposed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labelling. DNA labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. DEP-trapped microbeads can be measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. In this study, it was aimed to reveal how DNA length affects DEP characteristic of DNA-labeled microbeads. Dielectrophoretic crossover from the negative to the positive was measured for microbeads labeled with DNA length in 204 bp, 391 bp and 796 bp. After theoretical fitting of DEP crossover data, it was revealed that the surface conductance increased when the length o f labeled DNA increased. (More)

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Paper citation in several formats:
Ding, Z.; Kasahara, H.; Nakano, M. and Suehiro, J. (2015). Dielectrophoretic Characteristics of Microbeads Labeled with DNA of Various Lengths. In Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2015) - BIODEVICES; ISBN 978-989-758-071-0; ISSN 2184-4305, SciTePress, pages 185-189. DOI: 10.5220/0005280701850189

@conference{biodevices15,
author={Zhenhao Ding. and Hiromichi Kasahara. and Michihiko Nakano. and Junya Suehiro.},
title={Dielectrophoretic Characteristics of Microbeads Labeled with DNA of Various Lengths},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2015) - BIODEVICES},
year={2015},
pages={185-189},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005280701850189},
isbn={978-989-758-071-0},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2015) - BIODEVICES
TI - Dielectrophoretic Characteristics of Microbeads Labeled with DNA of Various Lengths
SN - 978-989-758-071-0
IS - 2184-4305
AU - Ding, Z.
AU - Kasahara, H.
AU - Nakano, M.
AU - Suehiro, J.
PY - 2015
SP - 185
EP - 189
DO - 10.5220/0005280701850189
PB - SciTePress

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