Authors:
Hendrian Dwikoloso Soebagjo
;
Fitria Kusumastuti
;
Parma Ratna Jaya
;
Susy Fatmariyanti
;
Endang Retnowati
and
Ugroseno S Yudho Bintoro
Affiliation:
Universitas Airlangga, Indonesia
Keyword(s):
Poorly differentiated retinoblastoma, NK cells, allogeneic, apoptosis, cancer immunotherapy
Abstract:
Introduction: Retinoblastoma is a retinal malignant tumor because of mutations on chromosome 13q14. This is associated with apoptosis deregulation processes. Both autologous or allogeneic NK cells play their roles in both innate and adaptive immune systems. They can stimulate apoptosis through several mechanisms. Methods: This study determined the role of allogeneic NK cells in retinoblastoma cell apoptosis. Poorly differentiated retinoblastoma tissues were tested with SDS-PAGE. Allogeneic NK cells were isolated from peripheral blood of the healthy family members of patients. There were two culture groups consisting of retinoblastoma cells only as the control and retinoblastoma treated with allogeneic NK cells as the treated group. Each group consisted of 10 well plates. Examinations of the expression of Bcl-2, Caspase-3, and Apoptosis were performed by the flowcytometry method. Result: The SDS PAGE electrophoresis tests showed strong expression of proteins with molecular weights of
14, 19, 28, 35, 53 and 85 kDa. The percentage of early-phase apoptosis is higher than late phase. There were significant correlations (α <0.05) between Bcl-2, Caspase-3, and the ratio of both on cell apoptosis. Conclusion: In conclusion, allogeneic NK cells play a role in retinoblastoma cell apoptosis, especially in the early phase. It is expected to be one of the new strategies in cancer immunotherapy
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