DETECTION OF ISOLATED NEMATODES IN CLUTTER ENVIRONMENTS USING SHAPE FEATURE HISTOGRAMS
Daniel Ochoa, Sidharta Gautama, Boris Vintimilla
2006
Abstract
We present an approach for detection of isolated Caenohabditis Elegans nematodes in clutter environments. The method is based on shape feature histograms which describe the distribution of features of second-order derivative responses of linear image structures. The shape features are able to distinguish isolated from overlapping nematodes and clutter, thereby improving the automated image analysis of nematode populations where accurate assessment of shape is needed. An evaluation is performed on a database of manually segmented images. Shape continuity features proved to have the highest discriminative power. This is consistent with the morphological structure of this kind of organism. Our experiments suggest that similar techniques can be used for identification of other linear shaped biological objects.
References
- Amri Muhammad, Karim Abdul, Roysam Badrinath, Dowell-Mesfin Natalie M., Jeromin Andreas, Yuksel Murat, Kalyanaraman Shivkumar, 2005. Automatic Selection of Parameters for Vessel/Neurite Segmentation Algorithms. IEEE transactions on Image processing, 14,1338 - 1350.
- Aylward S. R. and Bullitt E., 2002. Initialization, noise, singularities, and scale in height ridge traversal for tubular object centerline extraction. IEEE Trans. Med. Imag., 21, 61-75.
- Baek J., Cosman P., Baek J., Feng Z.,Silver J., Schafer W. 2002. Using machine vision to analyze and classify C Elegant behavioral phenotypes quantitatively. Journal of Neuroscience Methods, 118, 9-21.
- Cootes TF., Taylor C.J., Cooper C.H., 1995. Active shape model: their training and application. Computer Vision and Image understanding,6, 38-59.
- Fdez-Valdivia J., Perez De la Blanca N., Castilllo P., Gomez-Barcina A., 1992. Detecting nematode features from Digital Images. Journal of Nematology, 24,289-298.
- Gagaudakis G., Rosin P. ,2002 Incorporating shape into histograms for CBIR. Pattern Recognition, 35, 81-91.
- Geusebroek J., Smeulders A., Geerts H., 2001. A minimum cost approach for segmenting networks of lines. International Journal of Computer Vision, 43, 99-111.
- Hicks Y., Marshall D., Martin R.R., Rosin P.L., Bayer M.M., Mann D.G., 2002. Automatic landmarking for biological shape model. Proceedings International Conference on Image Processing, 2. 801-804.
- Kirbas Cemil and Quek Francis K.H., 2003. Vessel Extraction Techniques and Algorithms : A Survey. Proceedings of the Third IEEE Symposium on BioInformatics and BioEngineering, 238-246.
- Palhares L. ,Bastos R., 1997. A preliminary study for constructing a computational procedure for nematodes identification based on morphological aspects. European Conference for information Technology in Agriculture, 1,15-18
- Steger C., 1998 An unbiased detector of curvilinear structures. IEEE Trans. Pattern Anal Machine Intell., 20, 113-125.
- Twining C. J., Taylor C. J., 2001. Kernel Principal Component Analysis and the Construction of NonLinear Active Shape Models. In BMVC 2001, British Machine Vision Conference.
- Van Osta P., Geusebroek J.M., Ver Donck K., Bols L., Geysen J., ter Haar Romeny B.M., 2002. The principles of scale space applied to structure and colour in light microscopy. Proceedings Royal Microscopical Society, 37, 161-166.
Paper Citation
in Harvard Style
Ochoa D., Gautama S. and Vintimilla B. (2006). DETECTION OF ISOLATED NEMATODES IN CLUTTER ENVIRONMENTS USING SHAPE FEATURE HISTOGRAMS . In Proceedings of the First International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, ISBN 972-8865-40-6, pages 486-491. DOI: 10.5220/0001365504860491
in Bibtex Style
@conference{visapp06,
author={Daniel Ochoa and Sidharta Gautama and Boris Vintimilla},
title={DETECTION OF ISOLATED NEMATODES IN CLUTTER ENVIRONMENTS USING SHAPE FEATURE HISTOGRAMS},
booktitle={Proceedings of the First International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP,},
year={2006},
pages={486-491},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001365504860491},
isbn={972-8865-40-6},
}
in EndNote Style
TY - CONF
JO - Proceedings of the First International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP,
TI - DETECTION OF ISOLATED NEMATODES IN CLUTTER ENVIRONMENTS USING SHAPE FEATURE HISTOGRAMS
SN - 972-8865-40-6
AU - Ochoa D.
AU - Gautama S.
AU - Vintimilla B.
PY - 2006
SP - 486
EP - 491
DO - 10.5220/0001365504860491