SINGLE PARTICLE DETECTION - A Diagnostic Tool for Particle Associated Diseases like Alzheimer’s Disease and Creutzfeldt-Jakob Disease

Eva Birkmann, Susanne Aileen Funke, Detlev Riesner, Dieter Willbold

2008

Abstract

Neurodegenerative diseases like Alzheimer’s disease (AD), prion diseases and others are progressive and lethal. High-molecular weight aggregates of the Amyloid-β-peptides (A) or of the misfolded prion protein (PrP) are found in patients afflicted by AD or prion diseases, respectively. Despite of many attempts, neither a therapy for recovery, nor an early diagnosis at preclinical stages are available. Psychological tests and imaging approaches not directly related with a secure disease marker are in use only for late stages of the disease. The Creutzfeldt-Jakob-disease (CJD), a human prion disease, is caused by accumulation of aggregates consisting of an abnormally shaped version of PrP. CJD is diagnosed with certainty only by neuropathology post mortem. In this study a multidisciplinary development of a novel mode of single particle counting of immobilized Aβ and PrP aggregates as the most direct biomarkers for Alzheimer’s disease and Prion diseases, respectively, is introduced. For ultrasensitive detection of aggregates, the suitable instrumentation as well as data acquisition and data analysis are developed using single molecule detection and advanced laser scanning fluorescence techniques. In the novel assay development effort biochemistry, detection and analysis were improved to detect single aggregates immobilised on a surface. First results show the improvement of single particle detection of PrP-aggregates of TSE-afflicted cattle and hamsters as well as synthetic Aβ-aggregates.

Paper Citation

in Harvard Style

Birkmann E., Aileen Funke S., Riesner D. and Willbold D. (2008). SINGLE PARTICLE DETECTION - A Diagnostic Tool for Particle Associated Diseases like Alzheimer’s Disease and Creutzfeldt-Jakob Disease . In Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 2: BIOSIGNALS, (BIOSTEC 2008) ISBN 978-989-8111-18-0, pages 431-436. DOI: 10.5220/0001065104310436

in Bibtex Style

@conference{biosignals08,
author={Eva Birkmann and Susanne Aileen Funke and Detlev Riesner and Dieter Willbold},
title={SINGLE PARTICLE DETECTION - A Diagnostic Tool for Particle Associated Diseases like Alzheimer’s Disease and Creutzfeldt-Jakob Disease},
booktitle={Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 2: BIOSIGNALS, (BIOSTEC 2008)},
year={2008},
pages={431-436},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001065104310436},
isbn={978-989-8111-18-0},
}

in EndNote Style

TY - CONF
JO - Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 2: BIOSIGNALS, (BIOSTEC 2008)
TI - SINGLE PARTICLE DETECTION - A Diagnostic Tool for Particle Associated Diseases like Alzheimer’s Disease and Creutzfeldt-Jakob Disease
SN - 978-989-8111-18-0
AU - Birkmann E.
AU - Aileen Funke S.
AU - Riesner D.
AU - Willbold D.
PY - 2008
SP - 431
EP - 436
DO - 10.5220/0001065104310436