ELECTRICAL CHARACTERIZATION OF SEQUENCE-SPECIFIC LABEL-FREE DNA BY USING POLYSILICON WIRE

You-Lin Wu, Po-Yen Hsu, Chung-Ping Hsu, Wen- Cheng Liu

2010

Abstract

In this paper we report the electrical measurement of sequence-specific and label-free deoxyribonucleic acid (DNA) by using poly-silicon (poly-Si) wire. Four single-strained (ss) DNA bases, adenine (A), thymine (T), cytosine (C) and guanines (G), as well as double-stranded (ds) DNA sequences, ds(A-T) and ds(C-G), with different lengths and concentrations were dropped onto the poly-Si wire surface, and currents flowing through the poly-Si wire channel were determined. It is found that the amount change of the channel current I for ssDNAs with fixed length and fixed concentration is that T > C > G > A. For dsDNA sequence, we observe that ds(A-T) has higher I than does ds(C-G). We also prove that single base change in ssDNA is feasible by using the poly-Si wire sensor.

References

  1. de-los-Snatos-Alvarez, P., Lobo-Castanon M. Juses, Miranda-Ordieres, A. J., Tunon-Blanco, (2004). Current strategies for electrochemical detection of DNA with solid electrodes. Anal. Bioanal.Chem., 378, 104-118.
  2. Fritz, J., Cooper, E. B., Gaudet, S., Sorger, P. K. Manalis, S. R. (2002). Electronic detection of DNA by its intrinsic molecular charge. Proc. Natl. Acad. Sci. U.S.A., 99, 14142-14146.
  3. Hahm, J. I., & Lieber, C. M. (2001). Direct ultrasensitive electrical detection of DNA and DNA sequence variations using nanowire nanosensors. Nano lett., 4, 51-54.
  4. Kim, K. S., Ahn, S. K., Lee, Y., Lee, J. M., Roh, Y. (2006). Effects of gate voltage on the characteristics of sourcedrain current formed through DNA molecules. Thin Solid Films, 515, 822-826.
  5. Lander, E. S. (1999). Array of hope. Nat Genet. 21, 3-4.
  6. Mastrangelo, C. H., (1999). DNA analysis system on a chip. Solid-State Chemical and Biochemical Sensors, Techna Faenza., 495-476.
  7. Storm, A. J., van Noort, J., de Vries, S. Dekker, C. (2001). Insulating behaviour for DNA molecules between nanoelectrodes at the 100nm length scale. Appl. Phys. Lett., 79, 3881.
  8. Wang, J. (2000). Towards Genoelectronics: Electrochemical Biosensing of DNA Hybridization Chem. Eur. J., 5, 1681-1685.
  9. Wu, Y. L., Hsu, P. Y., Hu, S. H., Hung, W. C., Lin, S. T., Hsu, C. P., Cullis, A. G. (2008). Polysilicon Wire Sensor for Biochemical Detection with The Help of Capillary Atomic-Force-Microscopy Tip for Solution Transfer. 9th International conference on Solid State and Integrated Circuit technology. IEEE press.
  10. Xu, M., Endres, R. Arakawa, G., Y. (2007). The Electronic Properties of DNA bases, Small, 3, 1539- 1543.
  11. Zwolak, M., Di Ventra, M., (2008). Colloquium: Physical approaches to DNA sequencing and detection. Rev. Mod. Phys., 80, 141-165.
Download


Paper Citation


in Harvard Style

Wu Y., Hsu P., Hsu C. and Liu W. (2010). ELECTRICAL CHARACTERIZATION OF SEQUENCE-SPECIFIC LABEL-FREE DNA BY USING POLYSILICON WIRE . In Proceedings of the Third International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2010) ISBN 978-989-674-017-7, pages 170-173. DOI: 10.5220/0002712601700173


in Bibtex Style

@conference{biodevices10,
author={You-Lin Wu and Po-Yen Hsu and Chung-Ping Hsu and Wen- Cheng Liu},
title={ELECTRICAL CHARACTERIZATION OF SEQUENCE-SPECIFIC LABEL-FREE DNA BY USING POLYSILICON WIRE},
booktitle={Proceedings of the Third International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2010)},
year={2010},
pages={170-173},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002712601700173},
isbn={978-989-674-017-7},
}


in EndNote Style

TY - CONF
JO - Proceedings of the Third International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2010)
TI - ELECTRICAL CHARACTERIZATION OF SEQUENCE-SPECIFIC LABEL-FREE DNA BY USING POLYSILICON WIRE
SN - 978-989-674-017-7
AU - Wu Y.
AU - Hsu P.
AU - Hsu C.
AU - Liu W.
PY - 2010
SP - 170
EP - 173
DO - 10.5220/0002712601700173