Versatile Low-cost Modular Microfluidic Arrays for Cancer Diagnostics

James F. Rusling, Colleen Krause, Brunah Otieno, Karteek Kadimisetty, Chi Tang, Abhay Vaze, Gregory Bishop

2014

Abstract

Accurate, sensitive, multiplexed detection of biomarker proteins in serum and tissue holds significant promise for personalized cancer diagnostics and therapy monitoring. Here we describe fabrication details of a modular microfluidic system featuring a small chamber for on-line protein capture from serum by magnetic beads, positioned upstream of a nanostructured multi-sensor array chamber to achieve high sensitivity for up to eight proteins, with the ability to expand to many more proteins. Microfluidic chambers are made by templating PDMS channels on machined aluminum molds to avoid lithography, and mounted in hard plastic housings equipped with inlet and outlet lines and interfaced with valves. Gold immunoarrays fabricated by screen or ink-jet printing, or wet chemical etching of gold films utilize amperometry or electrochemiluminescence (ECL) detection. These arrays are interfaced with microfluidics to achieve well-controlled mass transport leading to excellent signal/noise and unprecedented sensitivities. With interest in low cost point of care (POC) systems, we developed a module to also facilitate automated microfluidic reagent and sample delivery utilizing an open source microcontroller and micropumps, with ECL detection by camera.

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Paper Citation


in Harvard Style

F. Rusling J., Krause C., Otieno B., Kadimisetty K., Tang C., Vaze A. and Bishop G. (2014). Versatile Low-cost Modular Microfluidic Arrays for Cancer Diagnostics . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2014) ISBN 978-989-758-013-0, pages 72-76. DOI: 10.5220/0004707400720076


in Bibtex Style

@conference{biodevices14,
author={James F. Rusling and Colleen Krause and Brunah Otieno and Karteek Kadimisetty and Chi Tang and Abhay Vaze and Gregory Bishop},
title={Versatile Low-cost Modular Microfluidic Arrays for Cancer Diagnostics},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2014)},
year={2014},
pages={72-76},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0004707400720076},
isbn={978-989-758-013-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2014)
TI - Versatile Low-cost Modular Microfluidic Arrays for Cancer Diagnostics
SN - 978-989-758-013-0
AU - F. Rusling J.
AU - Krause C.
AU - Otieno B.
AU - Kadimisetty K.
AU - Tang C.
AU - Vaze A.
AU - Bishop G.
PY - 2014
SP - 72
EP - 76
DO - 10.5220/0004707400720076