VIRUMILK - Biosensor for CMV Detection in Breast Milk from Lactating Women of Preterm Infants Less than 33 Weeks

S. Py, B. Wacogne, L. Pazart, A. Coaquette, W. Boireau, G. Herbein, G. Thiriez

2015

Abstract

Cytomegalovirus (CMV) is the leading cause of neonatal viral infection and can have a significant impact on the neurosensory development of newborns and especially preterm infants. While congenital CMV infection affects about 2-5% of very preterm infants, the risk of postnatal infection, particularly through breast milk, is much higher in this population (20%). However, infection could be considerably reduced by an early and fast screening of breast milk. Indeed, a treatment (freezing or pasteurization) of contaminated breast milk only could eliminate the virus. The idea of this position paper is that breast milk screening would help defining an appropriate and personalized feeding strategy. We explain how to develop a CMV biosensor to detect the virus in milk. It employs specific CMV antibodies grafted on a biochip surface to capture viral material and additional detection antibodies in a “sandwich assay” type system. Detection is based on optical absorption. It will be tested with a device developed previously. However, preliminary results obtained in ELISA technique with breast milk and homemade antibodies are presented in this position paper. The ulterior motive of this work is the fabrication of an autonomous and automated device that will be experimented in subsequent diagnosis strategy trial.

References

  1. Bevot, A., et al., 2012. Long-Term Outcome in Preterm Children with Human Cytomegalovirus Infection Transmitted via Breast Milk. Acta Paediatrica, 101 (4), 167-172.
  2. Bruchard, M., et al., 2013. Chemotherapy-Triggered Cathepsin B Release in Myeloid-Derived Suppressor Cells Activates the Nlrp3 Inflammasome and Promotes Tumor Growth. Nature Medicine, 19 (1), 57-64.
  3. Buxmann, H., et al., 2009. Incidence and Clinical Outcome of Cytomegalovirus Transmission via Breast Milk in Preterm Infants </=31 Weeks. Acta Paediatrica, 98 (2), 270-276.
  4. Chang, J.C., et al., 2013. Influence of Prolonged Storage Process, Pasteurization, and Heat Treatment on Biologically-Active Human Milk Proteins. Pediatrics and Neonatology, 54 (6), 360-366.
  5. Charrière, K., et al., 2012. SmarTTransfuser - A Biochip System for the Final ABO Compatibility Test, in: SciTePress - Science and Technology Publications, Vilamoura, Portugal, 257-262.
  6. Forsgren, M., 2004. Cytomegalovirus in Breast Milk: Reassessment of Pasteurization and Freeze-Thawing. Pediatric Research, 56 (4), 526-528.
  7. Goelz, R., et al., 2013. Long-Term Cognitive and Neurological Outcome of Preterm Infants with Postnatally Acquired CMV Infection through Breast Milk. Archives of Disease in Childhood. Fetal and Neonatal Edition, 98 (5), 430-433.
  8. Hamele, M., et al., 2010. Severe Morbidity and Mortality with Breast Milk Associated Cytomegalovirus Infection. The Pediatric Infectious Disease Journal, 29 (1), 84-86.
  9. Hamprecht, K., et al., 2008. Cytomegalovirus transmission to preterm infants during lactation. Journal of Clinical Virology, 41 (3), 198-205.
  10. Hamprecht, K., et al., 2004. Cytomegalovirus (CMV) Inactivation in Breast Milk: Reassessment of Pasteurization and Freeze-Thawing. Pediatric Research, 56 (4), 529-535.
  11. Hamprecht, K., et al., 2003. Rapid detection and quantification of cell free cytomegalovirus by a highspeed centrifugation-based microculture assay: comparison to longitudinally analyzed viral DNA load and pp67 late transcript during lactation. Journal of Clinical Virology, 28 (3), 303-316.
  12. Hayashi, S., et al., 2011. Transmission of cytomegalovirus via breast milk in extremely premature infants. J Perinatol, 31 (6), 440-445.
  13. Kurath, S., et., 2010. Transmission of Cytomegalovirus via Breast Milk to the Prematurely Born Infant: A Systematic Review. Clinical Microbiology and Infection, 16 (8), 1172-1178.
  14. Lanzieri, T., et al., 2013. Breast Milk-Acquired Cytomegalovirus Infection and Disease in VLBW and Premature Infants. Pediatrics, 131 (6), 1937-1945.
  15. Lombardi, G., et al., 2012. Breast Milk-Acquired Cytomegalovirus Infection in Very Low Birth Weight Infants. The Journal of Maternal-Fetal & Neonatal Medicine, 25 Suppl 3, 57 62.
  16. Mangeat, T., et al., 2011, Detection of the cytomegalovirus: a mobile device and a disposable cartridge for detection at the patient's bed. Conférence, Biodevices 2011, 26-29 January, Rome, Italy, In Proceedings of the International Conference on Biomedical Electronics and Devices, 103-108.
  17. Remy-Martin, F., et al., 2012. Surface Plasmon Resonance Imaging in Arrays Coupled with Mass Spectrometry (SUPRA-MS): Proof of Concept of on-Chip Characterization of a Potential Breast Cancer Marker in Human Plasma. Analytical and Bioanalytical Chemistry, 404 (2): 423-432.
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Paper Citation


in Harvard Style

Py S., Wacogne B., Pazart L., Coaquette A., Boireau W., Herbein G. and Thiriez G. (2015). VIRUMILK - Biosensor for CMV Detection in Breast Milk from Lactating Women of Preterm Infants Less than 33 Weeks . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015) ISBN 978-989-758-071-0, pages 115-120. DOI: 10.5220/0005251801150120


in Bibtex Style

@conference{biodevices15,
author={S. Py and B. Wacogne and L. Pazart and A. Coaquette and W. Boireau and G. Herbein and G. Thiriez},
title={VIRUMILK - Biosensor for CMV Detection in Breast Milk from Lactating Women of Preterm Infants Less than 33 Weeks },
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015)},
year={2015},
pages={115-120},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005251801150120},
isbn={978-989-758-071-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015)
TI - VIRUMILK - Biosensor for CMV Detection in Breast Milk from Lactating Women of Preterm Infants Less than 33 Weeks
SN - 978-989-758-071-0
AU - Py S.
AU - Wacogne B.
AU - Pazart L.
AU - Coaquette A.
AU - Boireau W.
AU - Herbein G.
AU - Thiriez G.
PY - 2015
SP - 115
EP - 120
DO - 10.5220/0005251801150120