Amperometric Urea Sensor - Enzyme Immobilization into Adjustable Membrane and Mathematical Characterization of the Biosensor

Julija Razumiene, Ieva Sakinyte, Vidute Gureviciene, Karolis Petrauskas

2015

Abstract

The prototype of amperometric biosensor for urea determination was designed. The enzyme electrode, made of a specially developed modified graphite (MG) paste, was produced by covering the electrode surface with adjustable membrane containing immobilized urease from Canavalia ensiformis (E.C. 3.5.1.5.). Simple methodology of urea determination in real time has been proposed. The experimental study and the mathematical model of the biosensor action have been performed.

References

  1. Amatore, C., Oleinick, A. Svir, I., da Mota, N., Thouin, L., 2006. Theoretical modeling and optimization of the detection performance: A new concept for electrochemical detection of proteins in microfluidic channels, Nonlinear Anal. Model. Control, 11, pp. 345-365.
  2. Baronas, R., Ivanauskas, F., Kulys, J 2010. Mathematical Modeling of Biosensors; Springer Series on Chemical Sensors and Biosensors, Springer: Dordrecht. Netherlands, vol 9.
  3. Budriene, S., Romaskevic, T., Pielichowski, K., Pielichowski, J., 2007. Synthesis and characterization of polyurethane microspheres and their application for immobilization of maltogenase. Polym. Adv. Technol., vol. 18, no. 1, pp. 67-71.
  4. Jensen, P. S., Bak, J., Ladefoged, S., Andersson-Engels, S., Friis-Hansen, L., 2004. Online monitoring of urea concentration in dialysate with dual-beam Fouriertransform near-infrared spectroscopy. Journal of Biomedical Optics, vol. 9, no. 3, pp. 553-557.
  5. Laurinavicius, V., Razumiene, J., Gureviciene, V., 2013. Bioelectrochemical conversion of urea on Carbon Black Electrode and Application. IEEE Sensors Journal, vol. 13, no. 6, pp. 2208-2213.
  6. Miglior, F., Sewalem, A., Jamrozik, J,. Bohmanova J., Lefebre, D.M., Motore, R.K., 2007. Genetic Analysis of Milk Urea Nitrogen and Lactose and Their Relationships with Other Production Traits in Canadian Holstein Cattle. J. Dairy Sc., vol. 90, no. 5, pp. 2468-2479.
  7. Patton, C.J., Crouch,. S.R., 1977. Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia. Anal. Chem., vol. 49, no. 3, pp. 464-469.
  8. Razumiene, J., Sakinyte, I. Kochane, T. Maciulyte, S., Straksys, A., Budriene, S., Barkauskas J., 2013. Carbon Electrode based Urea Sensor - Modification of Graphite and New Polymeric Carriers for Enzyme Immobilization. Proceedings of the BIODEVICES 2013, 6th International Conference on Biomedical Electronics and Devices, pp. 197-201. Available from: SCITEPRESS.
  9. Sant, W., Temple-Boyer, P., Chanié, E., Launay, J., Martinez, A. 2011. On-line monitoring of urea using enzymatic field effect transistors. Sensors and Actuators B: Chemical, vol. 160, no. 1, pp. 59-64.
  10. Soldatkin, O. O., Kucherenko, I.S., Marchenko, S. V., Ozansoy Kasap, B., Akata, B., Soldatkin, A. P., Dzyadevych, S. V., 2014. Application of enzyme/zeolite sensor for urea analysis in serum. Materials Science and Engineering: C, vol., no. 42, pp. 155-160.
  11. Voitechovic, E., Razumiene, J., Sakinyte, E., Barkauskas, J., 2010. Investigation of bioelectrocatalytic systems with PQQ-dependent GDH and carbonaceous materials. Biologija, vol. 56, no.1-4, pp. 83-87.
Download


Paper Citation


in Harvard Style

Razumiene J., Sakinyte I., Gureviciene V. and Petrauskas K. (2015). Amperometric Urea Sensor - Enzyme Immobilization into Adjustable Membrane and Mathematical Characterization of the Biosensor . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015) ISBN 978-989-758-071-0, pages 144-149. DOI: 10.5220/0005274501440149


in Bibtex Style

@conference{biodevices15,
author={Julija Razumiene and Ieva Sakinyte and Vidute Gureviciene and Karolis Petrauskas},
title={Amperometric Urea Sensor - Enzyme Immobilization into Adjustable Membrane and Mathematical Characterization of the Biosensor},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015)},
year={2015},
pages={144-149},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005274501440149},
isbn={978-989-758-071-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015)
TI - Amperometric Urea Sensor - Enzyme Immobilization into Adjustable Membrane and Mathematical Characterization of the Biosensor
SN - 978-989-758-071-0
AU - Razumiene J.
AU - Sakinyte I.
AU - Gureviciene V.
AU - Petrauskas K.
PY - 2015
SP - 144
EP - 149
DO - 10.5220/0005274501440149