Evaluation of Blood Glucose Level and Microscopic Pancreatic Islets of Langerhans Treated with Lawsonia Inermis Linnaeus Leaves Ethyl Acetate Extract in Streptozotocin-induced Diabetic Rat

Dwi Rita Anggraini, Tri Widyawati, Siti Syarifah, Arlinda Sari Wahyuni

2018

Abstract

Lawsonia inermis Linnaeus leaf, is one of alternative medicine that used to treat diabetes mellitus (DM) in Indonesia. We investigated the effect of ethyl acetate extract of Lawsonia inermis Linnaeus (EAE) on blood glucose level (BGL) and the histopathological alterations of pancreatic islets of Langerhans (iL) in streptozotocin (STZ)-induced diabetic rats. A number of 24 rats were divided into 6 groups, Normal control (NC) was feed ad libitum, while STZ-induced diabetic rats (SDR) groups were treated with normal saline 10 ml/kg (P1), glibencamide 10 mg/kg (P2), EAE 250 (P3), 500 (P4) and 1000 (P5) mg/kgbw, daily orally for 14 days. Data were analyzed using one way ANOVA followed by Dunnett t test. BGL of NC-(79.75 ± 4.95); P2 (77.5 ± 3.8); P3 (79.5±6.9); P4 (78.5 ± 4.5); P5 (83.25 ± 4.3) were lower than P1-treated groups (292.5 ±4.63) mg/dl, significantly (P<0.01). The histopathological evaluation showed that the perimeter of the islets of Langerhans P1 were shrinked (8.59 ± 1.8)µm, while P2(12.71 ± 5.4); P3 (11.42 ± 2.9); P4 (1679 ± 11;4) and P5 (14.37±3.5)µm were larger and closed to NC (19.27 ± 3.5)µm. The present study concluded that EAE have antihyperglycemic activy and improve the pancreatic islet of Langerhans structure.

Paper Citation

in Harvard Style

Anggraini D., Widyawati T., Syarifah S. and Wahyuni A. (2018). Evaluation of Blood Glucose Level and Microscopic Pancreatic Islets of Langerhans Treated with Lawsonia Inermis Linnaeus Leaves Ethyl Acetate Extract in Streptozotocin-induced Diabetic Rat. In Proceedings of the 3rd International Conference of Computer, Environment, Agriculture, Social Science, Health Science, Engineering and Technology - Volume 1: ICEST, ISBN 978-989-758-496-1, pages 108-112. DOI: 10.5220/0010039101080112

in Bibtex Style

@conference{icest18,
author={Dwi Rita Anggraini and Tri Widyawati and Siti Syarifah and Arlinda Sari Wahyuni},
title={Evaluation of Blood Glucose Level and Microscopic Pancreatic Islets of Langerhans Treated with Lawsonia Inermis Linnaeus Leaves Ethyl Acetate Extract in Streptozotocin-induced Diabetic Rat},
booktitle={Proceedings of the 3rd International Conference of Computer, Environment, Agriculture, Social Science, Health Science, Engineering and Technology - Volume 1: ICEST,},
year={2018},
pages={108-112},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010039101080112},
isbn={978-989-758-496-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 3rd International Conference of Computer, Environment, Agriculture, Social Science, Health Science, Engineering and Technology - Volume 1: ICEST,
TI - Evaluation of Blood Glucose Level and Microscopic Pancreatic Islets of Langerhans Treated with Lawsonia Inermis Linnaeus Leaves Ethyl Acetate Extract in Streptozotocin-induced Diabetic Rat
SN - 978-989-758-496-1
AU - Anggraini D.
AU - Widyawati T.
AU - Syarifah S.
AU - Wahyuni A.
PY - 2018
SP - 108
EP - 112
DO - 10.5220/0010039101080112