Isolation and Screening of Antagonistic Diazotrophic Endophytic Bacteria from Oil Palm Roots against Ganoderma boninense

Yurnaliza, Meylisa Purba, Dita Isnaini Rambe, Lista Sarimunggu, Isnaini Nurwahyuni, Sovia Lenny

2018

Abstract

Ganoderma boninense was pathogenic fungi affected rot disease on the basal stem of oil palm. Strategies to control G.boninense should be increased to reduced disease incidence. The aims of the study were to obtain isolates of bacterial endophyte diazotroph from oil palm roots and to examine their growth inhibiting activity to G. boninense. Oil palm roots were collected from three locations of oil palm plantation, namely Universitas Sumatera Utara (USU), PTPN IV Adolina, and community’s oil palm plantations at Desa Bingkat, Serdang Bedagai. Bacterial endophytes was isolated from sterile roots and cultivated on Nutrien Agar and Ashby's specific medium. Dual culture methods were used as antagonistic test to G.boninense. Potential isolates were identified by Vitek test analyzer. The results showed that population of the bacteria were abundant in non-commercial plantation (USU and community plantation). Screening of bacterial isolates to inhibit G.boninense colony resulted 13 isolates of bacterial with various of percentage inhibition growth ranged from 11 to 62.5%. One of potential isolate of bacteria performed red color and by Vitek analyzer was identified as Seratia marcencens.

Paper Citation

in Harvard Style

Yurnaliza., Purba M., Rambe D., Sarimunggu L., Nurwahyuni I. and Lenny S. (2018). Isolation and Screening of Antagonistic Diazotrophic Endophytic Bacteria from Oil Palm Roots against Ganoderma boninense.In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR, ISBN 978-989-758-449-7, pages 73-77. DOI: 10.5220/0010088700730077

in Bibtex Style

@conference{icosteerr18,
author={Yurnaliza and Meylisa Purba and Dita Isnaini Rambe and Lista Sarimunggu and Isnaini Nurwahyuni and Sovia Lenny},
title={Isolation and Screening of Antagonistic Diazotrophic Endophytic Bacteria from Oil Palm Roots against Ganoderma boninense},
booktitle={Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,},
year={2018},
pages={73-77},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010088700730077},
isbn={978-989-758-449-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,
TI - Isolation and Screening of Antagonistic Diazotrophic Endophytic Bacteria from Oil Palm Roots against Ganoderma boninense
SN - 978-989-758-449-7
AU - Yurnaliza.
AU - Purba M.
AU - Rambe D.
AU - Sarimunggu L.
AU - Nurwahyuni I.
AU - Lenny S.
PY - 2018
SP - 73
EP - 77
DO - 10.5220/0010088700730077