The Activity of Virgin Coconut Oil to Increase Proliferation and COX-2 Expression towards NIH 3T3 Cell Line

Jansen Silalahi, Dian Ika Perbina Meliala, Yuandani, Linda Margata, Denny Satria

2018

Abstract

This research aims to investigate the effect of VCO (Virgin Coconut Oil) to increase the activity of NIH 3T3 cell proliferation and COX-2 expression. The sample used were VCO, and acid value was determined. Proliferation was appraised using the MTT method. Furthermore, wound healing activity assays were established with a microscopic system, and expression of COX-2 was determined using RT-PCR. Acid value and % FFA of VCO are 1.07±0.01 and 0.51±0.02. VCO 62.5µg/mL with viability cells were 107.758±0.45%; 104.45±0.48% and 104.45±0.48% after 24h, 48h, and 72 h incubation, cell migration in the wound healing assay after 24 h and 48 h incubation (49.11±0.09% and 74.82±0.22%), and increase in expression of COX-2 (Control=1; and VCO=1.21). The results explain that VCO supply potent proliferation output. This study is planned to appraise of wound closure activity of VCO on the scratched monolayer of NIH 3T3 cell line.

Paper Citation

in Harvard Style

Silalahi J., Meliala D., Yuandani., Margata L. and Satria D. (2018). The Activity of Virgin Coconut Oil to Increase Proliferation and COX-2 Expression towards NIH 3T3 Cell Line.In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR, ISBN 978-989-758-449-7, pages 778-781. DOI: 10.5220/0010088907780781

in Bibtex Style

@conference{icosteerr18,
author={Jansen Silalahi and Dian Ika Perbina Meliala and Yuandani and Linda Margata and Denny Satria},
title={The Activity of Virgin Coconut Oil to Increase Proliferation and COX-2 Expression towards NIH 3T3 Cell Line},
booktitle={Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,},
year={2018},
pages={778-781},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010088907780781},
isbn={978-989-758-449-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,
TI - The Activity of Virgin Coconut Oil to Increase Proliferation and COX-2 Expression towards NIH 3T3 Cell Line
SN - 978-989-758-449-7
AU - Silalahi J.
AU - Meliala D.
AU - Yuandani.
AU - Margata L.
AU - Satria D.
PY - 2018
SP - 778
EP - 781
DO - 10.5220/0010088907780781