Isolation and Detection of Escherichia Coli Trimethoprim Resistance Gene from Layer Chickens in East Java Province, Indonesia by Polymerase Chain Reaction

Emy Koestanti Sabdoningrum, Sri Hidanah, Sri Chusniati, Wiwik Misaco, Retno Sri Wahyuni, Laras Retno Kinasih Harianto

2018

Abstract

Aim: The aim of this research was to detect the presence of a gene which is responsible for Escherichia coli trimethoprim resistance (TEM gene) using Polymerase Chain Reaction from layer chickens in East Java Province, Indonesia. Methods: Purposive sampling of 60 infundibulums has been done in laying chicken farms in those indicating Colibacillosis from 6 districts in East Java Province, Indonesia including Sidoarjo, Mojokerto, Jombang, Kediri, Bojonegoro and Blitar. Ten samples were collected from each district. Samples were isolated using Eosin Methylene Blue Agar and five biochemical tests of Sulfide Indol Motility (SIM), Simons Citrate Agar (SCA), Triple Sugar Iron Agar (TSIA), Urea Agar (UA) and Sugar Test were used for bacteria identification. The DNA was then isolated from Escherichia coli-positive samples prior to Polymerase Chain Reaction (PCR) amplification to investigate TEM gene that indicated trimethoprim antibiotic resistance. Result: Bacteriological tests showed that 30 of 60 samples (50%) were E. coli positive. Ten of those were from Blitar, 10 from Bojonegoro, 5 from Sidoarjo, and 5 from Jombang. Two samples from Kediri and Mojokerto districts were negative. From PCR amplification showed that 28 of 30 samples were negative due to TEM gene, and there were 2 positive samples shown from Bojonegoro and Blitar. This indicated that 6.66% of total samples were positive containing TEM gene. Conclusion: Based on bacteriological examination 50% of samples from six districts in East Java, Indonesia were E. coli positive, and 6.66% were trimethoprim resistant based on occurrence of TEM gen in PCR amplification.

Paper Citation

in Harvard Style

Koestanti Sabdoningrum E., Hidanah S., Chusniati S., Misaco W., Sri Wahyuni R. and Retno Kinasih Harianto L. (2018). Isolation and Detection of Escherichia Coli Trimethoprim Resistance Gene from Layer Chickens in East Java Province, Indonesia by Polymerase Chain Reaction.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 292-300. DOI: 10.5220/0007541602920300

in Bibtex Style

@conference{icps18,
author={Emy Koestanti Sabdoningrum and Sri Hidanah and Sri Chusniati and Wiwik Misaco and Retno Sri Wahyuni and Laras Retno Kinasih Harianto},
title={Isolation and Detection of Escherichia Coli Trimethoprim Resistance Gene from Layer Chickens in East Java Province, Indonesia by Polymerase Chain Reaction},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={292-300},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007541602920300},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Isolation and Detection of Escherichia Coli Trimethoprim Resistance Gene from Layer Chickens in East Java Province, Indonesia by Polymerase Chain Reaction
SN - 978-989-758-348-3
AU - Koestanti Sabdoningrum E.
AU - Hidanah S.
AU - Chusniati S.
AU - Misaco W.
AU - Sri Wahyuni R.
AU - Retno Kinasih Harianto L.
PY - 2018
SP - 292
EP - 300
DO - 10.5220/0007541602920300