The Effect of Macrophage Dose to Secretion Interleukin 6 (IL-6) on Model of Tuberculosis Granuloma In Vitro

Imam Agus Faizal, Agung Dwi Wahyu Widodo, Jusak Nugraha

2018

Abstract

Granuloma is a pathological sign of host response as a system of defense against infection of Mycobacterium tuberculosis (Mtb) causing Tuberculosis (TB) disease in humans estimated to be 8.7 million new cases, 1.4 million deaths, and about 2 billion latent infections. Macrophages are responsible for activating protective immune responses in innate and adaptive immune to control or eliminate infection. The inner body protects against Mtb infection producing various secretions of cytokines interleukin 6 (IL-6) that play the role of activating multinucleated giant cells, differentiation of macrophage T cells and thus stimulating CD4+ and CD8+ T-cells to strengthen macrophage antimicrobial capacity as early response reactions (early phase). The aim of this research is to see the effect of dose of macrophage on secretion and IL-6 expression on a TB granuloma model in vitro. Human blood was made by Peripheral Blood Mononuclear Cell (PBMC) and treated with the addition of a dose of macrophages of 1x105 cells/well, 2x105 cells/well and 3x105 cells/well and control (without macrophages). Then the bacterium Mycobacterium tuberculosis was added and then observing the secretion The enzyme-linked immunosorbent assay (ELISA) method of IL-6 cytokine during the 1st, 2nd, 3rd, 4th, and 5th days. The results of IL-6 examination on ELISA obtained p value of 0.7520 (p> 0.05). The conclusion of the study was that there was no effect of adding macrophage dose to cytokine secretion of IL-6 levels on granuloma TB model in vitro.

Paper Citation

in Harvard Style

Agus Faizal I., Dwi Wahyu Widodo A. and Nugraha J. (2018). The Effect of Macrophage Dose to Secretion Interleukin 6 (IL-6) on Model of Tuberculosis Granuloma In Vitro.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 306-311. DOI: 10.5220/0007541803060311

in Bibtex Style

@conference{icps18,
author={Imam Agus Faizal and Agung Dwi Wahyu Widodo and Jusak Nugraha},
title={The Effect of Macrophage Dose to Secretion Interleukin 6 (IL-6) on Model of Tuberculosis Granuloma In Vitro},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={306-311},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007541803060311},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - The Effect of Macrophage Dose to Secretion Interleukin 6 (IL-6) on Model of Tuberculosis Granuloma In Vitro
SN - 978-989-758-348-3
AU - Agus Faizal I.
AU - Dwi Wahyu Widodo A.
AU - Nugraha J.
PY - 2018
SP - 306
EP - 311
DO - 10.5220/0007541803060311