The Effects of Environmental Temperature Exposure of Blood Spatter Towards Protein and Agglutinations Level in ABO Blood Typing

Amanovitasari D., Kahar K., Prasetya R.A., Lestari T.P., Yudianto A.

2018

Abstract

The time needed for gathering evidence of a crime is an obstacle in the process of identifying the blood type in the blood spots on the cloth. The blood will dry out after contact with the outside air within 3-5 minutes and change the color from red to dark brown. Hence, the aim of this study was to investigate the effect of exposure to environmental temperature on the levels of protein and agglutination in ABO blood typing of blood spots after 0 (1 hour after blood drops on cloth), 5, 10, 15, and 20 days. Time series design was used in this study using 30 blood spots on cotton from 1 individual subject (blood type A). Blood samples were incubated at room temperature (23-24o C) (15 samples) and other ones were incubated at ambient temperature (exposed to sunlight and rain). Determination of protein level was performed with a UV spectrophotometer using trizole reagent. The agglutination level was examined by elution absorption method using antisera A and read macroscopically. One Way ANOVA and Kruskal Wallis test were performed in this trial and conclude that there was no significant effect to protein level (p>0.01). Based on the trial ABO blood typing can still be performed for all blood ages (0, 5, 10, 15, 20 days).

Paper Citation

in Harvard Style

D. A., K. K., R.A. P., T.P. L. and A. Y. (2018). The Effects of Environmental Temperature Exposure of Blood Spatter Towards Protein and Agglutinations Level in ABO Blood Typing.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 329-332. DOI: 10.5220/0007542303290332

in Bibtex Style

@conference{icps18,
author={Amanovitasari D. and Kahar K. and Prasetya R.A. and Lestari T.P. and Yudianto A.},
title={The Effects of Environmental Temperature Exposure of Blood Spatter Towards Protein and Agglutinations Level in ABO Blood Typing},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={329-332},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007542303290332},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - The Effects of Environmental Temperature Exposure of Blood Spatter Towards Protein and Agglutinations Level in ABO Blood Typing
SN - 978-989-758-348-3
AU - D. A.
AU - K. K.
AU - R.A. P.
AU - T.P. L.
AU - A. Y.
PY - 2018
SP - 329
EP - 332
DO - 10.5220/0007542303290332