Effect of Tumor Necrosis Factor Alpha (Tnf-A) And Interleukin-10 (II-10) Levels of Aggressive Periodontitis In Rats (Rattus Norvegicus) Induced by Agrregitibacter Actinomycetemcomitans

Dwi Leni Yuliana, Yoes Prijatna Dachlan, Heny Arwati

2018

Abstract

Aggregatibacter actinomycetemcomitans (A.actinomycetemcomitans) is a gram negative and a major bacterial agent associated with aggressive periodontitis in young adults. These bacteria are an important factor in pathogenesis of aggressive periodontitis. A. actinomycetemcomitans possesses fimbriae with an adhesin protein that was is the first bacterial molecules to make physical contact with host. A complex network of pro- and anti-inflammatory cytokines act in inflamed periodontal tissues. Among other cytokines, interleukin-10 (IL-10) is an important multifunctional cytokine. An increase or decrease in IL-10 levels caused by bacterial infection is critical for the individual control of balance between inflammatory, humoral and microbial challenges. Tumor necrosis factor-α (TNF-α) plays an important role in periodontal inflammation as it has substantial potential to increase bone resorption and is involved in connective tissue degradation by stimulating prostaglandin-E2 and colagenase. The purpose of this research was to analyze TNF-α and IL-10 levels of aggressive periodontitis in rats (Rattus norvegicus) induced by A. actinomycetemcomitans. This research was a true experimental study with Post Test Only Control Group Design. Rats were divided into 4 groups for 0,25; 0.5 and 0.75 CFU/mL, and negative control. Each group contained 5 rats. Aggressive periodontitis in rats was induced by injecting A.actinomycetemcomitans, at 48 hrs and 96 hrs post injection the inflammatory signs were observed, thee days later plasma were then collected to measure TNF- dan IL-10 level by ELISA. Analysis of Variance (ANOVA) showed significantly increase levels of TNF-α in the infected group compared with that of the control group. Aggressive periodontitis in rats showed by redness, abscess and tooth mobility. This condition indicated that A.actinomycetemcomitans has the ability to adhere and invade the periodontal tissue further producing a colony that caused periodontitis. High plasma level of TNF was seen in rats infected with 0.75 CFU/mL OF ac, while IL-10 was low as seen in rats infected with 0.5 CFU/mL OF Ac.

Paper Citation

in Harvard Style

Yuliana D., Dachlan Y. and Arwati H. (2018). Effect of Tumor Necrosis Factor Alpha (Tnf-A) And Interleukin-10 (II-10) Levels of Aggressive Periodontitis In Rats (Rattus Norvegicus) Induced by Agrregitibacter Actinomycetemcomitans.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 343-350. DOI: 10.5220/0007542603430350

in Bibtex Style

@conference{icps18,
author={Dwi Leni Yuliana and Yoes Prijatna Dachlan and Heny Arwati},
title={Effect of Tumor Necrosis Factor Alpha (Tnf-A) And Interleukin-10 (II-10) Levels of Aggressive Periodontitis In Rats (Rattus Norvegicus) Induced by Agrregitibacter Actinomycetemcomitans},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={343-350},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007542603430350},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Effect of Tumor Necrosis Factor Alpha (Tnf-A) And Interleukin-10 (II-10) Levels of Aggressive Periodontitis In Rats (Rattus Norvegicus) Induced by Agrregitibacter Actinomycetemcomitans
SN - 978-989-758-348-3
AU - Yuliana D.
AU - Dachlan Y.
AU - Arwati H.
PY - 2018
SP - 343
EP - 350
DO - 10.5220/0007542603430350