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Quality and Sensitifity of Presumtive Blood Test of Chicken (Gallus
gallus domesticus) Blood Using Leuco Malachite Green (LMG)
Nadia Yohana
1
, Bilqisthi Ari Putra
1
, Farah Aidah Nurreza
1
Djoko Legowo
2
1
Forensic Science, Postgraduate School, Universitas Airlangga, Jl. Airlangga No. 4-6, Surabaya, East Java, Indonesia
60115
2
Veterinary Pathology Department, Faculty of Veterinary Medicine,Universitas Airlangga, C Campus Mulyorejo,
Surabaya, East Java, Indonesia 60115
Keywords: Forensic, LMG test, blood, chicken
Abstract: Forensic Science is a branch of medical science that contains the application and utilization of science for law
enforcement and justice. Forensic laboratories have the task of supporting a component of case investigation,
identifying the case investigation component, the causes of death, known properties and signs for the benefit
of proof. The function of blood in the scene of the crime scene is to prove that it is blood of the victim or
blood that is not human blood. This research used Leuco Malachite Green (LMG) test. This study aims to
determine the quality of LMG reaction in chicken blood with different blood dilution. This research material
is LMG solution, chicken blood, aquadest solution. After blood is taken and dilution is performed 10-1 to 10-
5. The results of this study were obtained on the dilution of 10-1 obtained positive results (+), dilution 10-2
obtained positive results (+), dilution 10-3 obtained positive results (+), dilution 10-4 and dilution 10-5
obtained negative result (-). The conclusion of this study for the dilution of 10-1 obtained positive results (+),
dilution 10-2 obtained positive results (+), dilution 10-3 obtained positive results (+), dilution 10-4 obtained
negative results (-), and 10-5 dilution has negative results (-).
1 INTRODUCTION
Forensic Science is a branch of medical science
that contains the application and utilization of science
for law enforcement and justice (Idries et al, 2011).
Blood is probably the most commonly searched body
fluid in forensic casework (Takayama, 1912).
The Takayama or Hemochromogen test is used by
some forensic laboratories as a confirmatory test for
blood. The enhancement reagents often provide
valuable aid in crime scene analysis and laboratory
examination: in fact, they are able to detect minute
traces of blood even when attempts have been made
to wash away the incriminating evidence (Takayama,
1912). Leuco Malachite Green (LMG), leuko-crystal
violet (LCV), Lumiscene, Bluestar Forensics,
Fluorescein, and Luminol are widely used for
presumptive testing in casework at crime scene
investigations and in the laboratories. A presumptive
test will indicate if a biological substance such as
blood is present in a stain found. In this case, is the
stain found at a crime scene is blood or not (James et
al, 2005).
Chicken blood has larger erythrocytes than
mammals but it is smaller than mammalian
erythrocytes. The erythrocyte size of ungags
generally ranges from 10.7 x 6.1μm to 15.8 x 10.2μm.
In erythrocyte Chicken mature are elliptical shape
with a nucleus position in the middle (Bijanti, 2010).
Blood detection in animals in a study similar to
that of chicken blood has been performed on fish
blood using fluorescein test, Bluestar ©,
phenolphthalein, Hemastix®. The conclusion that can
be taken that fluorescein found the best results (ie,
low false positive rate, detecting the highest
proportion of the real positive). So it can be informed,
fluorescein testing can be further investigated to
improve its application in fisheries research. The use
of fluorescein, the injury can be detected up to 5 hours
after the injury occurred and the application of once
fluorescein, there is fluorescein detected much less
after one hour (Colotelo, 2009).
Yohana, N., Putra, B., Nureza, F. and Legowo, D.
Quality and Sensitifity of Presumtive Blood Test of Chicken (Gallus gallus domesticus) Blood Using Leuco Malachite Green (LMG).
DOI: 10.5220/0007543003650367
In Proceedings of the 2nd International Conference Postgraduate School (ICPS 2018), pages 365-367
ISBN: 978-989-758-348-3
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2018 by SCITEPRESS – Science and Technology Publications, Lda. All rights reserved
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