The Difference of Chloride Levels from Blood Serum Centrifuged at

3662 Rpm Speed for 10 Minutes and 20 Minutes

Rima Merdaliani

, Indah Sari

and Maria Nuraeni

Forensic Science Depertement, University of Airlangga, Airlangga Street, Surabaya, Indonesia

Lecture, STIKesMuhammadiyah, Palembang, Indonesia

Lecture, University of Catholic Musi Charitas, Palembang, Indonesia

Keywords: Chloride, Centrifugation Speed, Centrifugation Time

Abstract: Chloride is the main anion of extracellular fluid. One of the methods that must be considered in pre-

analytical stage of chlorides processing the examination material. The method of chloride examination is

ISE (Ion Selective Electrode) by using Easylyte. This study aims to determine the difference of chloride

levels from blood serum centrifuged at 3662 rpm speed for 10 minutes and 20 minutes. This study is a pre-

experimental using a Static Group Comparison Design. The normal test result of centrifugation for 10

minutes is 0.921 > 0.05 and for 20 minutes is 0.203 > 0.05 which means that the data is normally

distributed. The result of statistical analysis of T-Test (paired T-Test) shows p = 0.125, and α = 0.025 (p>α).

It means that there is no difference between chloride levels from blood serum that is centrifuged at 3662

rpm speed for 10 minutes and 20 minutes. This study concludes that there is no significant difference of

chloride levels between centrifugation at 3662 rpm for 10 minutes and 20 minutes.

1 INTRODUCTION

Electrolytes are compounds that consist of positive

or negative charged solution. Most metabolisms

need electrolytes. Metabolisms are also affected by

electrolytes (Yaswir, 2012). According to Burtis et

al (2008), electrolytes mostly consist of anions and

cations. Anion electrolytes include Cl

, HCO

, while cations include Na

, K

, Ca

and

Chloride is the most common anion found in

extracellular fluid. Chloride is excreted and

reabsorbed along with sodium in the kidney. In

normal condition, on average adults consume 50–

200 mEq/L of chloride per day and excreted around

1-2 mEq/L of chloride through feces (Yaswir, 2012).

Chloride assessment is used to observe chloride and

other electrolyte levels. Chloride assessment

material is serum obtained from venipuncture. The

blood in test tubes were frozen at room temperature

for 20-30 minutes, then centrifuged at 3000 rpm for

5-15 minutes (KepMenKes No 1792, 2010).

Centrifugation speed used in specimen

processing was 1500 g with 10 cm rotor radius,

which was converted into 3622 rpm for 10 minutes

(WHO 2

revision, 2002). According to WHO, the

minimum centrifugation time in chloride

examination is 10 minutes. WHO does not state the

maximum time in chloride examination. Therefore,

the author selects 20 minutes as the time limit to

measure whether chloride level is still within normal

limit or not.

Centrifugation is a technique to separate

materials based on molecular weight under certain

speed. This separation technique is used to separate

or purify proteins, particles, or cellular organelles

sedimented according to their relative size and shape

(Bintang, 2010). This study employs Easylyte as a

device to examine chloride level in serum. The

principle of Easylyte in measuring sodium,

potassium, lithium chloride, calcium, pH, biological

fluid is an ion technology selective. Selective

electrodes develop variable voltages responded by

ion concentration. The correlation between

developed voltage and responsive ion concentration

was logarithmic.

368

Merdaliani, R., Sari, I. and Nuraeni, M.

The Difference of Chloride Levels from Blood Serum Centrifuge at 3662 RPM Speed for 10 Minutes and 20 Minutes.

DOI: 10.5220/0007543103680370

In Proceedings of the 2nd International Conference Postgraduate School (ICPS 2018), pages 368-370

ISBN: 978-989-758-348-3

This study aims to determine any differences of

chloride levels in serum centrifuged at 3662 rpm for

10 minutes and 20 minutes.

2 SUBJECTS AND METHODS

The samples were obtained from venous blood

through vacuum system as the following : Verify

preparation by recording medications consumed or

other preparation factors that may affect

examination such as diet and fasting, place needle in

the holder, place tourniquet at approximately 3-4

fingers above the cubital fossa. After the subjects

were asked to clench their hands, disinfect the skin

around the area with 70% alcohol pad and let it dry.

Position the holder with needle hole facing upwards.

Perform venipuncture by inserting needle to vein

lumen. Afterwards, the tourniquet is released.

Meanwhile, specimen processing procedure is as

follows: Blood specimens were left for 20-30

minutes at room temperature, then centrifugation

process was performed to separate serum from the

blood at 3662 rpm for 10 minutes and 20 minutes.

Sample analysis steps are as follows: place 100 µl

serum to serum cup, the device display will show,

“analyze blood?”, press “yes” then sample probe

will come down. Insert probe sample to serum cup

then press “yes”. Let the suctioning process finished,

then probe will come back up and the device will

analyze samples. Analysis results were shown on the

device’s display. Chloride level analysis was

performed using EasyLyte device with ISE (Ion

Selective Electrode) method (Riswanto, 2013).

3 RESULTS AND DISCUSSION

Based on chloride level analysis from serum

centrifuged for 10 and 20 minutes at 3662 rpm as

shown in Table 1, mean, minimum, maximum, and

standard deviation (SD) values were obtained.

Table 1. The Results of examination of chloride

concentrate for 10 minutes and 20 minutes.

Sample

Code

Concentrated

chloride levels

for 10 minutes

Concentrated

chloride levels

for 20 minutes

107.30

107.50

104.10

103.40

105.30

105.50

106.00

106.40

105.80

104.80

105.90

105.50

106.20

105.30

106.10

105.80

108.40

108.00

108.80

108.40

103.00

102.20

105.40

105.80

104.90

105.70

105.30

102.10

103.30

107.30

108.60

103.70

105.30

102.50

103.70

105.30

101.70

102.90

104.90

104.50

106.90

107.70

104.90

105.70

104.50

105.30

102.90

103.70

102.50

103.70

104.90

105.70

101.30

102.50

103.30

103.70

102.90

104.50

104.10

105.40

103.80

106.30

106.20

105.80

103.20

102.80

107.20

107.10

105.40

104.50

104.70

104.30

103.70

106.50

106.10

MEAN

104.90

105.09

MIN

101.30

102.20

MAX

108.80

108.60

1.7547

1.6019

Chloride level centrifuged for 10 minutes had an

average of 104.90 ± 1.7 mEq/L, minimum value of

101.30 mEq/L, and maximum value of 108.80

mEq/L. Meanwhile, chloride level centrifuged for 20

minutes showed mean value of 105.09 ± 1.6 mEq/L,

minimum value of 102.20 mEq/L, and maximum

value of 108.60 mEq/L.

The dependent variable in this study was

chloride level and the independent variable in this

study was serum centrifuged at 3662 rpm for 10 and

20 minutes. Chloride level analysis was performed

using ISE (Ion Selective Electrode) with Easylyte

automatic device that has been conducted through

method verification test, i.e. precision and accuracy.

The Difference of Chloride Levels from Blood Serum Centrifuge at 3662 RPM Speed for 10 Minutes and 20 Minutes

369

Method verification result in chloride analysis

showed that chloride analysis method can be used

because precision/CV value obtained was below

maximum CV (2%), both in within day precision

(0.3%) and between day precision (0.79%) and

accuracy/bias value (0.08%) obtained was below

maximum bias (8%). Results of chloride level in

blood serum centrifuged at 3662 rpm for 10 minutes

showed mean value of 104.90 mEq/L which was

close to chloride level in blood serum centrifuged at

3662 rpm for 20 minutes with mean value of 105.09

mEq/L. Statistical analysis using paired T-test

showed p value = 0.125 with α value = 0.025 (p >α)

which means that there was no significant difference

of chloride levels in blood serum centrifuged at 3662

rpm for 10 and 20 minutes.

4 SUGGESTION

Further studies are needed by using different

electrolyte parameters and variation of different

centrifugation speed and duration.

5 CONCLUSION

According to the result of chloride level analysis in

blood serum centrifuged at 3662 rpm for 10 minutes

and 20 minutes, this study concluded that there was

no difference of chloride levels in blood serum

centrifuged at 3662 rpm for 10 minutes and 20

minutes.

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