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The Difference of Chloride Levels from Blood Serum Centrifuged at
3662 Rpm Speed for 10 Minutes and 20 Minutes
Rima Merdaliani
1
, Indah Sari
2
and Maria Nuraeni
3
1
Forensic Science Depertement, University of Airlangga, Airlangga Street, Surabaya, Indonesia
2
Lecture, STIKesMuhammadiyah, Palembang, Indonesia
3
Lecture, University of Catholic Musi Charitas, Palembang, Indonesia
Keywords: Chloride, Centrifugation Speed, Centrifugation Time
Abstract: Chloride is the main anion of extracellular fluid. One of the methods that must be considered in pre-
analytical stage of chlorides processing the examination material. The method of chloride examination is
ISE (Ion Selective Electrode) by using Easylyte. This study aims to determine the difference of chloride
levels from blood serum centrifuged at 3662 rpm speed for 10 minutes and 20 minutes. This study is a pre-
experimental using a Static Group Comparison Design. The normal test result of centrifugation for 10
minutes is 0.921 > 0.05 and for 20 minutes is 0.203 > 0.05 which means that the data is normally
distributed. The result of statistical analysis of T-Test (paired T-Test) shows p = 0.125, and α = 0.025 (p>α).
It means that there is no difference between chloride levels from blood serum that is centrifuged at 3662
rpm speed for 10 minutes and 20 minutes. This study concludes that there is no significant difference of
chloride levels between centrifugation at 3662 rpm for 10 minutes and 20 minutes.
1 INTRODUCTION
Electrolytes are compounds that consist of positive
or negative charged solution. Most metabolisms
need electrolytes. Metabolisms are also affected by
electrolytes (Yaswir, 2012). According to Burtis et
al (2008), electrolytes mostly consist of anions and
cations. Anion electrolytes include Cl
+
, HCO
3
-
,
H
2
PO
4
2-
, while cations include Na
+
, K
+
, Ca
+
and
Mg
2+
.
Chloride is the most common anion found in
extracellular fluid. Chloride is excreted and
reabsorbed along with sodium in the kidney. In
normal condition, on average adults consume 50–
200 mEq/L of chloride per day and excreted around
1-2 mEq/L of chloride through feces (Yaswir, 2012).
Chloride assessment is used to observe chloride and
other electrolyte levels. Chloride assessment
material is serum obtained from venipuncture. The
blood in test tubes were frozen at room temperature
for 20-30 minutes, then centrifuged at 3000 rpm for
5-15 minutes (KepMenKes No 1792, 2010).
Centrifugation speed used in specimen
processing was 1500 g with 10 cm rotor radius,
which was converted into 3622 rpm for 10 minutes
(WHO 2
nd
revision, 2002). According to WHO, the
minimum centrifugation time in chloride
examination is 10 minutes. WHO does not state the
maximum time in chloride examination. Therefore,
the author selects 20 minutes as the time limit to
measure whether chloride level is still within normal
limit or not.
Centrifugation is a technique to separate
materials based on molecular weight under certain
speed. This separation technique is used to separate
or purify proteins, particles, or cellular organelles
sedimented according to their relative size and shape
(Bintang, 2010). This study employs Easylyte as a
device to examine chloride level in serum. The
principle of Easylyte in measuring sodium,
potassium, lithium chloride, calcium, pH, biological
fluid is an ion technology selective. Selective
electrodes develop variable voltages responded by
ion concentration. The correlation between
developed voltage and responsive ion concentration
was logarithmic.
368
Merdaliani, R., Sari, I. and Nuraeni, M.
The Difference of Chloride Levels from Blood Serum Centrifuge at 3662 RPM Speed for 10 Minutes and 20 Minutes.
DOI: 10.5220/0007543103680370
In Proceedings of the 2nd International Conference Postgraduate School (ICPS 2018), pages 368-370
ISBN: 978-989-758-348-3
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